Mutations in Genes Encoding Sorting Nexins Alter Production ofIntracellular and Extracellular Proteases in Aspergillus nidulans
Mutations in Genes Encoding Sorting Nexins Alter Production ofIntracellular and Extracellular Proteases in Aspergillus nidulans
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编码分选连接蛋白的基因突变改变构巢曲霉细胞内和细胞外蛋白酶的产生
DOI：
10.1534/genetics.108.095315
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发表时间：
2009
期刊：
影响因子：
3.3
通讯作者：
B. F. Cheetham
中科院分区：
文献类型：
--
作者：
M. Katz;C. Evans;Emma E Heagney;P. Vankuyk;J. Kelly;B. F. Cheetham
XprG是一种可能的P53样转录激活因子，调节细胞外蛋白水解酶的产生，以响应营养限制，并可能在细胞程序性死亡中发挥作用。为了确定可能参与XprG调控途径的基因，我们分离了xprG2回复株，并证明其携带突变基因，我们将其命名为SogA-C(xprG抑制因子)。SogA1突变体中的易位断裂点被定位在酿酒酵母VPS5的同源基因上，定位数据表明sogB与VPS17同源基因紧密连锁。SogA1和sogB1突变的互补以及sogA2和sogB1等位基因无义突变的鉴定证实了这一点。Vps17p和Vps5p是一个复合体的一部分，参与酵母中空泡蛋白的分选和哺乳动物细胞表面受体的调节。蛋白酶谱表明，SogA-C的突变允许分泌细胞内的蛋白酶，就像酿酒酵母vps5和vps17突变株一样。与酿酒酵母不同，突变株的胞内蛋白水解酶产量明显高于酿酒酵母。对丝氨酸蛋白酶基因表达的分析表明，存在一种XprG不依赖于XprG的机制来调节胞外蛋白水解酶基因的表达，以响应碳饥饿，并在假复原菌中被激活。
XprG, a putative p53-like transcriptional activator, regulates production of extracellular proteases in response to nutrient limitation and may also have a role in programmed cell death. To identify genes that may be involved in the XprG regulatory pathway, xprG2 revertants were isolated and shown to carry mutations in genes which we have named sogA-C (suppressors of xprG). The translocation breakpoint in the sogA1 mutant was localized to a homolog of Saccharomyces cerevisiae VPS5 and mapping data indicated that sogB was tightly linked to a VPS17 homolog. Complementation of the sogA1 and sogB1 mutations and identification of nonsense mutations in the sogA2 and sogB1 alleles confirmed the identification. Vps17p and Vps5p are part of a complex involved in sorting of vacuolar proteins in yeast and regulation of cell-surface receptors in mammals. Protease zymograms indicate that mutations in sogA-C permit secretion of intracellular proteases, as in S. cerevisiae vps5 and vps17 mutants. In contrast to S. cerevisiae, the production of intracellular protease was much higher in the mutants. Analysis of serine protease gene expression suggests that an XprG-independent mechanism for regulation of extracellular protease gene expression in response to carbon starvation exists and is activated in the pseudorevertants.
影响因子：
3.3
作者：
Horazdovsky, BF;Davies, BA;Emr, SD
通讯作者：
Emr, SD
影响因子：
4.8
作者：
Fuster, Jose J.;Gonzalez, Jose M.;Andres, Vicente
通讯作者：
Andres, Vicente
影响因子：
56.9
作者：
Chu, S;DeRisi, J;Herskowitz, I
通讯作者：
Herskowitz, I