Convergent extension requires adhesion-dependent biomechanical integration of cell crawling and junction contraction.
Convergent extension requires adhesion-dependent biomechanical integration of cell crawling and junction contraction.
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DOI：
10.1016/j.celrep.2022.110666
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发表时间：
2022-04-26
期刊：
影响因子：
8.8
通讯作者：
Wallingford, John B.
中科院分区：
文献类型：
--
作者：
Weng, Shinuo;Huebner, Robert J.;Wallingford, John B.
会聚延伸（CE）是一种进化上保守的集体细胞运动，在发育过程中延伸几个器官系统。研究揭示了两种不同的细胞机制，一种基于细胞爬行，另一种基于连接收缩。这两种行为是否相互配合尚不清楚。在这里，使用活细胞成像，我们表明，爬行和收缩的行为都是独立和联合的，但CE是更有效的，当它们通过机械相互作用集成。因此，我们开发了一个计算模型，同时考虑爬行和收缩。该模型概括了整合这两种模式的生物力学功效，并进一步阐明了细胞粘附如何影响这两种模式及其整合。最后，我们使用这些见解来了解一个未充分研究的连环蛋白，Arvcf，在CE的功能。这些数据是重要的提供有趣的生物力学和细胞生物学的见解，涉及人类神经管缺陷和骨骼发育不良的基本形态发生过程。细胞爬行和连接收缩被认为是两种不同的机制，用于在胚胎发育过程中的会聚延伸延伸。Weng等人证明，这两种模式不仅一起工作，而且还合作以获得更好的疗效。还开发了一个计算模型来模拟这种合作。
Convergent extension (CE) is an evolutionarily conserved collective cell movement that elongates several organ systems during development. Studies have revealed two distinct cellular mechanisms, one based on cell crawling and the other on junction contraction. Whether these two behaviors collaborate is unclear. Here, using live-cell imaging, we show that crawling and contraction act both independently and jointly but that CE is more effective when they are integrated via mechano-reciprocity. We thus developed a computational model considering both crawling and contraction. This model recapitulates the biomechanical efficacy of integrating the two modes and further clarifies how the two modes and their integration are influenced by cell adhesion. Finally, we use these insights to understand the function of an understudied catenin, Arvcf, during CE. These data are significant for providing interesting biomechanical and cell biological insights into a fundamental morphogenetic process that is implicated in human neural tube defects and skeletal dysplasias. Cell crawling and junction contraction are thought to be two distinct mechanisms used for elongation by convergent extension during embryonic development. Weng et al. demonstrate that the two modes not only work together but also collaborate for a better efficacy. A computational model is also developed to model this collaboration.
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影响因子：
11.8
作者：
Huebner, Robert J.;Weng, Shinuo;Lee, Chanjae;Sarikaya, Sena;Papoulas, Ophelia;Cox, Rachael M.;Marcotte, Edward M.;Wallingford, John B.
通讯作者：
Wallingford, John B.
影响因子：
0.7
作者：
Brodland, GW
通讯作者：
Brodland, GW
影响因子：
2.7
作者：
KELLER, R;TIBBETTS, P
通讯作者：
TIBBETTS, P
影响因子：
9.8
作者：
Finegan, Tara M.;Hervieux, Nathan;Sanson, Benedicte
通讯作者：
Sanson, Benedicte
DOI：
10.1126/science.1254211
发表时间：
2014-10-31
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Buckley CD;Tan J;Anderson KL;Hanein D;Volkmann N;Weis WI;Nelson WJ;Dunn AR
通讯作者：
Dunn AR