Fission yeast Lem2 and Man1 perform fundamental functions of the animal cell nuclear lamina.
Fission yeast Lem2 and Man1 perform fundamental functions of the animal cell nuclear lamina.
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DOI：
10.4161/nucl.18824
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发表时间：
2012-01
期刊：
影响因子：
--
通讯作者：
Sazer S
中科院分区：
文献类型：
--
作者：
Gonzalez Y;Saito A;Sazer S
在动物细胞中，核层由层粘连蛋白和层粘连蛋白相关蛋白组成，具有多种功能：它为核膜提供结构支架，并将蛋白质和异染色质连接到核周。在酵母中，蛋白质和包括端粒在内的大的异染色域也位于外围，但没有证据表明酵母有板层或纤维状核膜支架。然而，我们发现裂殖酵母的Lem2和Man1蛋白执行动物细胞层蛋白相关蛋白LAP2/Emerin/Man1(LEM)亚家族的进化远亲，执行动物细胞层的基本功能。这些完整的核内膜定位蛋白，与核内定位的DNA结合的螺旋-延伸-螺旋(HeH)结构域，影响核膜结构和完整性，对于核周端粒的丰富和通过其HEH结构域锚定染色质，很可能是转录抑制的异染色质，是必不可少的。这些数据表明，核层的核心功能在真菌和动物细胞之间是保守的，可以在分裂酵母中执行，而不需要核层或其他中间丝蛋白。
In animal cells the nuclear lamina, which consists of lamins and lamin-associated proteins, serves several functions: it provides a structural scaffold for the nuclear envelope and tethers proteins and heterochromatin to the nuclear periphery. In yeast, proteins and large heterochromatic domains including telomeres are also peripherally localized, but there is no evidence that yeast have lamins or a fibrous nuclear envelope scaffold. Nonetheless, we found that the Lem2 and Man1 proteins of the fission yeast Schizosaccharomyces pombe, evolutionarily distant relatives of the Lap2/Emerin/Man1 (LEM) sub-family of animal cell lamin-associated proteins, perform fundamental functions of the animal cell lamina. These integral inner nuclear membrane localized proteins, with nuclear localized DNA binding Helix-Extension-Helix (HEH) domains, impact nuclear envelope structure and integrity, are essential for the enrichment of telomeres at the nuclear periphery and by means of their HEH domains anchor chromatin, most likely transcriptionally repressed heterochromatin, to the nuclear periphery. These data indicate that the core functions of the nuclear lamina are conserved between fungi and animal cells and can be performed in fission yeast, without lamins or other intermediate filament proteins.
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影响因子：
4
作者：
Gonzalez, Yanira;Meerbrey, Kristen;Sazer, Shelley
通讯作者：
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影响因子：
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影响因子：
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作者：
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作者：
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影响因子：
7.8
作者：
Anderson, Daniel J.;Hetzer, Martin W.
通讯作者：
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