14-3-3epsilon contributes to tumour suppression in laryngeal carcinoma by affecting apoptosis and invasion.
14-3-3epsilon contributes to tumour suppression in laryngeal carcinoma by affecting apoptosis and invasion.
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DOI：
10.1186/1471-2407-10-306
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发表时间：
2010-06-19
期刊：
影响因子：
3.8
通讯作者：
Fu WN
中科院分区：
文献类型：
--
作者：
Che XH;Chen H;Xu ZM;Shang C;Sun KL;Fu WN
14-3-3epsilon 调节广泛的生物过程，包括细胞周期控制、增殖和凋亡，并在神经发生和恶性肿瘤的形成中发挥重要作用。然而，14-3-3ε在致癌过程中的确切功能和调控机制尚未阐明。通过RT-PCR和蛋白质印迹评估14-3-3ε的表达。分别通过Transwell迁移实验和MTT实验测定Hep-2细胞的侵袭力和活力。流式细胞术检测Hep-2细胞的细胞周期和凋亡。喉鳞状细胞癌（LSCC）组织中14-3-3ε mRNA和蛋白表达量显着低于手术切缘清晰组织。统计分析显示，转移淋巴结中的14-3-3ε蛋白水平低于配对肿瘤组织中的水平。此外，III期或IV期肿瘤中14-3-3ε的蛋白水平显着低于I期或II期肿瘤。与对照Hep-2细胞相比，14-3-3epsilon-GFP组和阴性对照GFP组的活细胞百分比分别为36.68±14.09%和71.68±12.10%。对照组、阴性对照GFP和对照组中S期比例分别为22.47±3.36%、28.17±3.97%和46.15±6.82%，亚G1期细胞凋亡比例分别为1.23±1.02%、2.92±1.59%和13.72±3.89%。分别为14-3-3ε-GFP组。对照组、阴性对照GFP组和14-3-3ε-GFP组的凋亡细胞百分比分别为0.84±0.25%、1.08±0.24%和2.93±0.13%。对照组、阴性对照GFP组和14-3-3epsilon-GFP组穿透滤膜的细胞数分别为20.65±1.94、17.63±1.04和9.1±0.24，表明不同组之间存在显着差异。 LSCC 组织中 14-3-3epsilon 表达的降低有助于 LSCC 的发生和进展。 14-3-3epsilon可以促进细胞凋亡并抑制LSCC的侵袭。
14-3-3epsilon regulates a wide range of biological processes, including cell cycle control, proliferation, and apoptosis, and plays a significant role in neurogenesis and the formation of malignant tumours. However, the exact function and regulatory mechanism of 14-3-3epsilon in carcinogenesis have not been elucidated. The expression of 14-3-3epsilon was assessed by RT-PCR and western blotting. The invasiveness and viability of Hep-2 cells were determined by the transwell migration assay and MTT assay, respectively. Cell cycle and apoptosis of Hep-2 cells were detected by flow cytometry. The mRNA and protein expression of 14-3-3epsilon in larynx squamous cell carcinoma (LSCC) tissues were significantly lower than those in clear surgical margin tissues. Statistical analysis showed that the 14-3-3epsilon protein level in metastatic lymph nodes was lower than that in paired tumour tissues. In addition, the protein level of 14-3-3epsilon in stage III or IV tumours was significantly lower than that in stage I or II tumours. Compared with control Hep-2 cells, the percentages of viable cells in the 14-3-3epsilon-GFP and negative control GFP groups were 36.68 ± 14.09% and 71.68 ± 12.10%, respectively. The proportions of S phase were 22.47 ± 3.36%, 28.17 ± 3.97% and 46.15 ± 6.82%, and the apoptotic sub-G1 populations were 1.23 ± 1.02%, 2.92 ± 1.59% and 13.72 ± 3.89% in the control, negative control GFP and 14-3-3epsilon-GFP groups, respectively. The percentages of the apoptotic cells were 0.84 ± 0.25%, 1.08 ± 0.24% and 2.93 ± 0.13% in the control, negative control GFP and 14-3-3epsilon-GFP groups, respectively. The numbers of cells that penetrated the filter membrane in the control, negative control GFP and 14-3-3epsilon-GFP groups were 20.65 ± 1.94, 17.63 ± 1.04 and 9.1 ± 0.24, respectively, indicating significant differences among the different groups. Decreased expression of 14-3-3epsilon in LSCC tissues contributes to the initiation and progression of LSCC. 14-3-3epsilon can promote apoptosis and inhibit the invasiveness of LSCC.
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DOI：
10.1053/paor.1999.0217
发表时间：
1999-01-01
期刊：
Pathology oncology research : POR
影响因子：
--
作者：
Du, B;Wang, P;Du, B
通讯作者：
Du, B
影响因子：
4.8
作者：
Nguyen, TA;Takemoto, LJ;Takemoto, DJ
通讯作者：
Takemoto, DJ
影响因子：
4.2
作者：
Liu, Jia;Guo, Yan;Fu, Wei-Neng
通讯作者：
Fu, Wei-Neng
影响因子：
4.8
作者：
Won, J;Kim, DY;Joe, CO
通讯作者：
Joe, CO
影响因子：
6.1
作者：
Shang Chao;Fu Wei-neng;Sun Kai-lai
通讯作者：
Sun Kai-lai