REGγ is associated with multiple oncogenic pathways in human cancers.
REGγ is associated with multiple oncogenic pathways in human cancers.
复制标题
DOI：
10.1186/1471-2407-12-75
复制
发表时间：
2012-02-23
期刊：
影响因子：
3.8
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
He J;Cui L;Zeng Y;Wang G;Zhou P;Yang Y;Ji L;Zhao Y;Chen J;Wang Z;Shi T;Zhang P;Chen R;Li X
最近的研究表明蛋白酶体激活剂REGγ在癌症进展中的作用。由于已知的REGγ靶点数量有限，因此尚不清楚哪些癌症和途径与REGγ相关。采用免疫组化（IHC）方法检测4种不同肿瘤组织中REGγ蛋白的表达。通过NCBI基因表达综合数据库（GEO）检索、芯片平台验证，统计分析REGγ在相应肿瘤中的差异表达。根据Pearson相关系数确定与REGγ高度相关的基因。功能链接通过Ingenuity Core分析进行估计。最后，通过建立的癌细胞系中的RT-PCR分析和人结肠癌组织中的IHC进行验证。在这里，我们通过微组织阵列分析证明了REGγ在四种不同癌症类型中的过表达。使用公开可用的微阵列数据库和生物学研究的荟萃分析，我们验证了四种类型癌症中REGγ基因表达的升高，并确定了与REGγ表达显著相关的基因，包括p53，Myc途径和多种其他癌症相关途径中的基因。预测的相关性与定量RT-PCR分析基本一致。这项研究为我们提供了REGγ基因表达谱及其与癌症中多种癌症相关途径的联系的新见解。我们的研究结果表明REGγ在多种癌症类型中具有潜在的重要致病作用，并暗示REGγ是推定的癌症标志物。
Recent studies suggest a role of the proteasome activator, REGγ, in cancer progression. Since there are limited numbers of known REGγ targets, it is not known which cancers and pathways are associated with REGγ. REGγ protein expressions in four different cancers were investigated by immunohistochemistry (IHC) analysis. Following NCBI Gene Expression Omnibus (GEO) database search, microarray platform validation, differential expressions of REGγ in corresponding cancers were statistically analyzed. Genes highly correlated with REGγ were defined based on Pearson's correlation coefficient. Functional links were estimated by Ingenuity Core analysis. Finally, validation was performed by RT-PCR analysis in established cancer cell lines and IHC in human colon cancer tissues Here, we demonstrate overexpression of REGγ in four different cancer types by micro-tissue array analysis. Using meta-analysis of publicly available microarray databases and biological studies, we verified elevated REGγ gene expression in the four types of cancers and identified genes significantly correlated with REGγ expression, including genes in p53, Myc pathways, and multiple other cancer-related pathways. The predicted correlations were largely consistent with quantitative RT-PCR analysis. This study provides us novel insights in REGγ gene expression profiles and its link to multiple cancer-related pathways in cancers. Our results indicate potentially important pathogenic roles of REGγ in multiple cancer types and implicate REGγ as a putative cancer marker.
登录
查看更多内容
前列腺癌的基因表达谱揭示了多个分子途径在转移过程中的参与。
影响因子：
3.8
作者：
Chandran, Uma R.;Ma, Changqing;Dhir, Rajiv;Bisceglia, Michelle;Lyons-Weiler, Maureen;Liang, Wenjing;Michalopoulos, George;Becich, Michael;Monzon, Federico A.
通讯作者：
Monzon, Federico A.
影响因子：
14.9
作者：
Jensen LJ;Kuhn M;Stark M;Chaffron S;Creevey C;Muller J;Doerks T;Julien P;Roth A;Simonovic M;Bork P;von Mering C
通讯作者：
von Mering C
影响因子：
11.5
作者：
Hong, Yi;Ho, Kok Sun;Cheah, Peh Yean
通讯作者：
Cheah, Peh Yean
影响因子：
5.8
作者：
Okamura, T;Taniguchi, SI;Shigemasa, C
通讯作者：
Shigemasa, C
影响因子：
4.4
作者：
Maouche, Seraya;Poirier, Odette;Godefroy, Tiphaine;Olaso, Robert;Gut, Ivo;Collet, Jean-Phillipe;Montalescot, Gilles;Cambien, Francois
通讯作者：
Cambien, Francois