Monoclonal antibody to human carcinoma-associated protein complex: quantitation in normal and tumor tissue.
Monoclonal antibody to human carcinoma-associated protein complex: quantitation in normal and tumor tissue.
复制标题
人类癌症相关蛋白复合物的单克隆抗体：正常组织和肿瘤组织中的定量。
作者：
R. Falcioni;A. Sacchi;James Resau;S. Kennel
我们已经用两种单克隆抗体（MoAb）（Cancer Res.，46：707-712，1986）。两种单克隆抗体之一，135- 13 C，识别培养中的几种人类癌上的TSP-180样蛋白复合物。MoAb 135- 13 C已用于从A431细胞纯化人TSP-180复合物，纯化的材料用于免疫F344大鼠以产生针对人TSP-180复合物的另一种MoAb 439- 9 B。该单克隆抗体不沉淀鼠TSP-180或与鼠细胞结合。MoAb 135- 13 C和439- 9 B从A431细胞沉淀相同的蛋白质，但不相互竞争结合位点，表明它们识别相同蛋白质上的不同表位。这两种MoAb已用于双位点测定，以定量人体细胞和组织上的TSP-180蛋白。癌细胞系A431、SW 948和A549均给出高值（46至443 ng/mg蛋白质），而鼠肿瘤、人黑素瘤和人成纤维细胞为阴性（小于10 ng/mg蛋白质）。在检测水平下（低于10 ng/mg蛋白质），2例正常个体尸检的大部分组织对人TSP-180呈阴性。一些器官有中等范围的表达：脾脏，5至111 ng/ml的蛋白质;结肠，24至111;和小肠，39至99。1例原发性结肠癌和1例喉肿瘤为阳性（144 - 372 ng/mg蛋白），而5例乳腺癌、1例胃肿瘤、1例转移性黑色素瘤和1例肾肿瘤为阴性。这些数据表明，人TSP-180可能优先表达在某些恶性肿瘤的不同来源。TSP-180作为肿瘤标志物的潜力需要进一步研究。
We have identified and quantitated a tumor protein complex, TSP-180, on murine carcinomas with two monoclonal antibodies (MoAbs) (Cancer Res., 46: 707-712, 1986). One of the two MoAbs, 135-13C, recognizes a TSP-180-like protein complex on several human carcinomas in culture. MoAb 135-13C has been used to purify the human TSP-180 complex from A431 cells and the purified material used to immunize F344 rats to produce another MoAb, 439-9B, to the human TSP-180 complex. This MoAb does not precipitate the murine TSP-180 or bind to murine cells. Both MoAb 135-13C and 439-9B precipitated the same proteins from A431 cells but did not compete with each other for binding sites, indicating that they recognize different epitopes on the same protein. The two MoAbs have been used in a two-site assay to quantitate TSP-180 proteins on human cells and tissues. Carcinoma cell lines A431, SW948, and A549 all give high values (46 to 443 ng/mg of protein) while murine tumors, a human melanoma, and human fibroblasts are negative (less than 10 ng/mg of protein). Most tissues from autopsy of 2 normal individuals are negative for human TSP-180 at the levels tested (less than 10 ng/mg of protein). Some organs have intermediate range expression: spleen, 5 to 111 ng/ml of protein; colon, 24 to 111; and small intestine, 39 to 99. One primary colon and one larynx tumor were positive (144 to 372 ng/mg of protein) while 5 breast carcinomas, a stomach tumor, a metastatic melanoma, and a kidney tumor were negative. These data indicate that human TSP-180 may be preferentially expressed in certain malignant carcinomas of diverse origin. The potential for TSP-180 as a tumor marker requires further study.
单克隆抗体定义的黑色素瘤中 GD2 和 GD3 抗原水平与抗体介导的细胞毒性之间的相关性。
影响因子：
11.2
作者：
Thurin,J;Thurin,M;Kimoto,Y;Herlyn,M;Lubeck,MD;Elder,DE;Smereczynska,M;Karlsson,KA;ClarkJr,WM;Steplewski,Z
通讯作者：
Steplewski,Z
新生霉素抑制紫外线和甲磺酸甲酯诱导的人成纤维细胞 DNA 损伤修复的研究。
影响因子：
14.9
作者：
Snyder,RD;VanHouten,B;Regan,JD
通讯作者：
Regan,JD