Super-resolution imaging using nano-bells.
Super-resolution imaging using nano-bells.
复制标题
DOI：
10.1038/s41598-018-34744-6
复制
发表时间：
2018-11-06
影响因子：
4.6
通讯作者：
Clark M
中科院分区：
文献类型：
--
作者：
Fuentes-Domínguez R;Pérez-Cota F;Naznin S;Smith RJ;Clark M
在本文中，我们展示了一种光学超分辨率的新方案，部分灵感来自 PALM 和 STORM。在该方案中，视野中的每个物体都标有信号，以便可以单独检测它们。通过这样做，我们可以以比衍射极限高得多的分辨率分别识别和定位每个物体。我们通过对显着小于光学分辨率极限的纳米粒子进行成像来证明这一点。在这种情况下，我们使用的“标签”是由金属纳米颗粒制成的纳米级“钟”的振动频率，其声振动频率在数GHz范围内。由于颗粒的振动很容易被激发和检测，并且频率与颗粒尺寸直接相关，因此我们可以从许多尺寸足够不同的颗粒中分离出信号，即使它们小于光学分辨率极限，并且分离距离小于光学分辨率极限。使用这种方案，我们能够以~3纳米的精度定位纳米粒子的位置。这具有许多潜在的优点——这种纳米颗粒很容易插入细胞并且耐受性良好，颗粒不会漂白，并且可以很容易地生产出尺寸非常分散的颗粒。我们估计使用金纳米球的振动频率可以在每个光点扩散函数中访问 50 个或更多不同的粒子（或频率通道）。然而，可以使用更复杂的结构（例如纳米棒）和检测技术（例如使用偏振或波长选择性检测）来访问更多通道，从而使该技术成为实现超光学分辨率成像的通用方法。
In this paper we demonstrate a new scheme for optical super-resolution, inspired, in-part, by PALM and STORM. In this scheme each object in the field of view is tagged with a signal that allows them to be detected separately. By doing this we can identify and locate each object separately with significantly higher resolution than the diffraction limit. We demonstrate this by imaging nanoparticles significantly smaller than the optical resolution limit. In this case the “tag” we have used is the frequency of vibration of nanoscale “bells” made of metallic nanoparticles whose acoustic vibrational frequency is in the multi-GHz range. Since the vibration of the particles can be easily excited and detected and the frequency is directly related to the particle size, we can separate the signals from many particles of sufficiently different sizes even though they are smaller than, and separated by less than, the optical resolution limit. Using this scheme we have been able to localise the nanoparticle position with a precision of ~3 nm. This has many potential advantages - such nanoparticles are easily inserted into cells and well tolerated, the particles do not bleach and can be produced easily with very dispersed sizes. We estimate that 50 or more different particles (or frequency channels) can be accessed in each optical point spread function using the vibrational frequencies of gold nanospheres. However, many more channels may be accessed using more complex structures (such as nanorods) and detection techniques (for instance using polarization or wavelength selective detection) opening up this technique as a generalized method of achieving super-optical resolution imaging.
登录
查看更多内容
影响因子：
4
作者：
Guillet, Yannick;Rossignol, Clement;Ravaine, Serge
通讯作者：
Ravaine, Serge
影响因子：
4.4
作者：
Hodak, JH;Martini, I;Hartland, GV
通讯作者：
Hartland, GV
影响因子：
48
作者：
Rust, Michael J.;Bates, Mark;Zhuang, Xiaowei
通讯作者：
Zhuang, Xiaowei
影响因子：
4.6
作者：
Pérez-Cota F;Smith RJ;Moradi E;Marques L;Webb KF;Clark M
通讯作者：
Clark M
影响因子：
29.4
作者：
Guerrero-Martinez, Andres;Perez-Juste, Jorge;Liz-Marzan, Luis M.
通讯作者：
Liz-Marzan, Luis M.