Mesenchymal influences on epithelial differentiation in developing systems
Mesenchymal influences on epithelial differentiation in developing systems
复制标题
间充质对发育系统上皮分化的影响
DOI：
10.1242/jcs.1988.supplement_10.15
复制
发表时间：
1988
影响因子：
4
通讯作者：
Mark W. J. Ferguson
中科院分区：
文献类型：
--
作者：
P. Sharpe;Mark W. J. Ferguson
摘要 间充质组织：细胞、基质和可溶性因子，影响多种胚胎上皮细胞的形态发生、增殖和分化，例如胚胎上皮细胞。存在于牙齿、皮肤、乳腺和唾液腺中。间充质衍生物也“维持”成体上皮细胞，例如口腔粘膜的局部增殖率和细胞角蛋白组成。这种上皮-间质相互作用的异常会导致多种病理，例如癌前病变，例如癌前病变。白斑、肿瘤和牛皮癣，而对此类相互作用的治疗操作可以防止牙种植体剥落。在所有这些系统中，在细胞和分子水平上了解间质如何向上皮发出信号以及上皮如何处理和响应此类信号至关重要。我们使用发育中的胚胎腭作为模型系统和重要器官来研究这些问题：间充质信号传导的失败会导致常见且令人痛苦的腭裂出生缺陷。双侧腭架由胚胎第 11 天 (E11) 小鼠的上颌突产生，最初沿着舌头两侧垂直生长，在 E13.8 上升高到舌背上方的水平位置，并在 E14 上在中线彼此融合。各架内侧缘上皮相互融合，形成中线上皮缝，基底上细胞死亡，基底（干）细胞合成细胞外基质分子，转变为间充质细胞。同时，口腔上皮分化为复层鳞状细胞，鼻上皮分化为假复层纤毛柱状细胞。口腔、内侧和鼻上皮的分化是由体内和体外的底层间充质决定的。信号传导涉及可溶性生长因子（例如生长因子）组合的分叉作用。腭上皮和间质上的 TGF-α、TGF-β、PDGF 和 FGF。这些因子刺激腭间充质细胞合成特定的细胞外基质分子，并刺激上皮细胞上此类分子的受体的出现。通过这种方式，间充质可溶性因子和细胞外基质分子的组合指导腭上皮分化。这些信号作用于上皮基底（干）细胞，使它们合成独特的蛋白质，这可能指导子细胞的后续分化。在最极端的例子中，即内侧边缘上皮，这些信号导致基底上皮细胞转化为间充质细胞，从而证明它们确实是多能干细胞。
Summary Mesenchyme tissue: cells, matrix and soluble factors, influence the morphogenesis, proliferation and differentiation of a variety of embryonic epithelia, e.g. in the tooth, skin, mammary and salivary glands. Mesenchyme derivatives also ‘maintain’ adult epithelia, e.g. the local proliferation rate and cytokeratin composition of oral mucosa. Abnormalities in such epithelial–mesenchymal interactions lead to a variety of pathologies such as premalignant lesions, e.g. leukoplakia, tumours and psoriasis, whilst therapeutic manipulation of such interactions can prevent the exfoliation of dental implants. In all of these systems it is critical to understand, at the cellular and molecular levels, how the mesenchyme signals to the epithelium and how the latter processes and responds to such signals. We have investigated such questions using the developing embryonic palate both as a model system and as an important organ: failure of mesenchymal signalling leads to the common and distressing birth defect of cleft palate. Bilateral palatal shelves arise from the maxillary processes of embryonic day 11 (E11) mice, grow initially vertically down the sides of the tongue, elevate on E13.8 to a horizontal position above the dorsum of the tongue and fuse with each other in the midline on E14. The medial edge epithelia of each shelf fuse with each other to form a midline epithelial seam, suprabasal cells die, and the basal (stem) cells synthesize extracellular matrix molecules and turn into mesenchymal cells. Simultaneously the oral epithelia differentiate into stratified squamous cells and the nasal epithelia into pseudostratified ciliated columnar cells. Oral, medial and nasal epithelial differentiation is specified by the underlying mesenchyme in vivo and in vitro. Signalling involves a bifurcating action of a combination of soluble growth factors e.g. TGF-α, TGF-β, PDGF and FGF on palatal epithelia and mesenchyme. These factors stimulate the synthesis of specific extracellular matrix molecules by palate mesenchyme cells, and the appearance of receptors for such molecules on epithelial cells. In this way, a combination of mesenchymal soluble factors and extracellular matrix molecules direct palatal epithelial differentiation. These signals act on epithelial basal (stem) cells, causing them to synthesize unique proteins, which may direct subsequent differentiation of daughter cells. In the most extreme example, namely the medial edge epithelia, these signals result in the basal epithelial cells transforming into mesenchymal cells, thus demonstrating that they are indeed multipotential stem cells.
登录
查看更多内容
甲状腺上皮细胞向间充质样细胞转化过程中，细胞骨架和甲状腺球蛋白表达发生变化。
DOI：
10.1242/dev.102.3.605
发表时间：
1988
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Greenburg,G;Hay,ED
通讯作者：
Hay,ED
DOI：
--
发表时间：
1976-10
期刊：
Journal of embryology and experimental morphology
影响因子：
--
作者：
R. Greene;R. Pratt
通讯作者：
R. Greene;R. Pratt
成人皮肤的表皮-真皮相互作用。
DOI：
10.1111/1523-1747.ep12544628
发表时间：
1982
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Briggaman,RA
通讯作者：
Briggaman,RA
成年小鼠皮肤和粘膜异型重组上皮中的角蛋白多肽模式。
DOI：
10.1111/j.1432-0436.1984.tb01388.x
发表时间：
1984
期刊：
Differentiation; research in biological diversity
影响因子：
--
作者：
Schweizer,J;Winter,H;Hill,MW;Mackenzie,IC
通讯作者：
Mackenzie,IC
DOI：
10.1073/pnas.84.21.7696
发表时间：
1987-11-01
影响因子：
11.1
作者：
LYNCH, SE;NIXON, JC;ANTONIADES, HN
通讯作者：
ANTONIADES, HN