Activity-dependent [Ca2+]i changes in guinea pig vagal motoneurons: relationship to the slow afterhyperpolarization.
Activity-dependent [Ca2+]i changes in guinea pig vagal motoneurons: relationship to the slow afterhyperpolarization.
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豚鼠迷走神经运动神经元的活动依赖性[Ca2]i变化：与缓慢后超极化的关系。
DOI：
10.1152/jn.1997.78.2.825
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Yarom,Y
中科院分区：
文献类型：
--
作者：
Lasser-Ross,N;Ross,WN;Yarom,Y
放大图片作者：William N. Ross和Yosef Yarom。豚鼠迷走神经运动神经元的活动依赖性[Ca 2 +]变化：与缓慢后超极化的关系。神经生理学杂志。78：825-834，1997。向豚鼠脑干切片中的迷走运动神经元注射荧光[Ca 2 +]指示剂fura-2、furaptra或Calcium Green-1。绿色-1。在索马和树突中测量棘波诱导的荧光变化，同时在索马中用尖锐微电极记录持久的后超极化。Na+尖峰或Ca 2+尖峰增加索马和树突中所有位置的[Ca 2 +]i（测量为指示剂荧光的变化）。当使用非饱和指示剂时，动作电位序列中的每个尖峰引起大约相等幅度的荧光的阶跃增加。所有位置的峰值变化均发生在最后一次动作电位时。用低浓度的钙绿色-1或furaptra测量的瞬变在细胞体中的恢复时间常数为500- 1，500 ms。树突的恢复时间比索马快。去甲肾上腺素敏感的缓慢后超极化（sAHP）的峰值时间为10800 ms，恢复时间常数为2-5 s，远长于荧光变化的恢复时间过程。在大鼠海马CA 1区的锥体神经元上重复这些实验中的一些，得到类似的结果。在这两种细胞类型中，数据表明sAHP的上升阶段和下降阶段的时间过程，也不是基础电导，直接反映了[Ca 2 +]变化的时间过程。这些参数之间的联系机制尚不清楚。一种可能性是涉及额外的第二信使系统。
Lasser-Ross, Nechama, William N. Ross, and Yosef Yarom.Activity-dependent [Ca2+]ichanges in guinea pig vagal motoneurons: relationship to the slow afterhyperpolarization.J. Neurophysiol.78: 825–834, 1997. Vagal motoneurons in slices from the guinea-pig brain stem were injected with the fluorescent [Ca2+]iindicators fura-2, furaptra, or Calcium Green-1. Spike-induced fluorescence changes were measured in the soma and dendrites and simultaneously the long-lasting afterhyperpolarization was recorded with a sharp microelectrode in the soma. Na+spikes or Ca2+spikes increased [Ca2+]i(measured as a change in indicator fluorescence) in all locations in the soma and dendrites. Each spike in a train of action potentials caused a step increase in fluorescence of about equal amplitude when nonsaturating indicators were used. Peak changes at all locations occurred at the time of the last action potential. Transients measured with low concentrations of Calcium Green-1 or furaptra had a recovery time constant of ∼500–1,500 ms in the cell body. The recovery time course was faster in the dendrites than in the soma. The norepinephrine-sensitive, slow afterhyperpolarization (sAHP) had a time to peak of ∼800 ms and a recovery time constant of 2–5 s, much longer than the recovery time course of the fluorescence changes. Some of these experiments were repeated on pyramidal neurons from the CA1 region of the rat hippocampus with similar results. In both cell types, the data suggest that the time course of neither the rising phase nor the falling phase of the sAHP, nor the underlying conductance, directly reflects the time course of the [Ca2+]ichange. The mechanism connecting the parameters remains unclear. One possibility is that an additional second messenger system is involved.
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