Ferric chloride-induced murine carotid arterial injury: A model of redox pathology.
Ferric chloride-induced murine carotid arterial injury: A model of redox pathology.
复制标题
DOI：
10.1016/j.redox.2012.11.001
复制
发表时间：
2013
期刊：
影响因子：
11.4
通讯作者：
Silverstein, Roy L.
中科院分区：
文献类型：
--
作者：
Li, Wei;McIntyre, Thomas M.;Silverstein, Roy L.
氯化铁（FeCl3）诱导的血管损伤是一种广泛使用的闭塞性血栓形成模型，报告了无菌封闭血管系统中血小板活化的情况。该模型基于氧化还原诱导的内皮细胞损伤，该模型简单，对抗凝血和抗血小板药物均敏感。血小板聚集阻塞血流所需的时间提供了与血栓性疾病病理相关的血管损伤的定量测量。我们改进了传统的fecl3诱导颈动脉模型，使数据具有高重复性和低变异。本文将描述我们的方法，并报告通过不同的FeCl3浓度和暴露来改变氧化损伤的作用。为了探索实验组之间的最大差异，可能需要调整所选择的FeCl3剂量和暴露时间。对大血管的氧化损伤足以诱发血栓形成。氧化损伤本身就是血栓形成的刺激因素。不同程度的血管壁氧化还原损伤诱导不同程度的血栓形成。闭塞性血栓形成时间是体内血管损伤的定量指标。
Ferric chloride (FeCl3) induced vascular injury is a widely used model of occlusive thrombosis that reports platelet activation in the context of an aseptic closed vascular system. This model is based on redox-induced endothelial cell injury, which is simple and sensitive to both anticoagulant and anti-platelets drugs. The time required for platelet aggregation to occlude blood flow gives a quantitative measure of vascular damage that is pathologically relevant to thrombotic disease. We have refined the traditional FeCl3-induced carotid artery model making the data highly reproducible with lower variation. This paper will describe our artifices and report the role of varying the oxidative damage by varying FeCl3 concentrations and exposure. To explore a maximum difference between experimental groups, adjustment of the selected FeCl3 dose and exposure duration may be necessary. ► Oxidative damage to large blood vessels is sufficient to induce thrombosis. ► Oxidative damage alone is a thrombotic stimulus. ► Different level of redox injury to the vessel wall induces varied grades of thrombosis. ► The time to occlusive thrombosis is a quantitative measure of in vivo vascular damage.
登录
查看更多内容
影响因子：
--
作者：
Vilahur G;Padro T;Badimon L
通讯作者：
Badimon L
影响因子：
7.5
作者：
Whinna, Herbert C.
通讯作者：
Whinna, Herbert C.
影响因子：
20.3
作者：
Wang, Li;Miller, Christine;Taubman, Mark B.
通讯作者：
Taubman, Mark B.
动脉粥样硬化的炎症。
DOI：
10.1161/atvbaha.108.179705
发表时间：
2012-09
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Libby P
通讯作者：
Libby P
影响因子：
20.1
作者：
Chen, Kan;Febbraio, Maria;Silverstein, Roy L.
通讯作者：
Silverstein, Roy L.