Knockout of the lignin pathway gene BnF5H decreases the S/G lignin compositional ratio and improves Sclerotinia sclerotiorum resistance in Brassica napus.
Knockout of the lignin pathway gene BnF5H decreases the S/G lignin compositional ratio and improves Sclerotinia sclerotiorum resistance in Brassica napus.
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敲除木质素途径基因 BnF5H 降低了甘蓝型油菜中的 S/G 木质素组成比并提高了核盘菌抗性
DOI：
10.1111/pce.14208
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发表时间：
2022-01
影响因子：
7.3
通讯作者：
Liu, Liezhao
中科院分区：
文献类型：
--
作者：
Cao, Yanru;Yan, Xingying;Ran, Shuyao;Ralph, John;Smith, Rebecca A.;Chen, Xueping;Qu, Cunmin;Li, Jiana;Liu, Liezhao
阿魏酸-5-羟化酶是被子植物中愈创木单酯醇转化为异戊二醇的关键酶。单倍比被认为影响生物量的稳定性和对植物病害的抗性。油菜菌核病是由油菜菌核病菌引起的一种重要病害，严重影响了油菜的生产。迄今为止，还没有关于木质素单体比例对抗S。B为菌核病。油菜。在B中，通过CRISPR/Cas9一致地敲除四个显性表达的阿魏酸-5-羟化酶基因。napus，并产生了三个突变体系。基于KO-7中的M柱染色和2D-NMR谱分析的结果，与野生型相比，S/G木质素组成比降低。对S.与野生型相比，三个f5 h突变系的茎和叶中的菌核增加。此外，我们还发现f5 h突变株系的茎强度显著增加。总的来说，我们首次证明了通过敲除F5 H基因降低S/G比可以改善S。B对菌核病的抗性。增加了茎的强度。油菜菌核病是影响油菜生产的主要病害之一，它能引起油菜茎叶腐烂，降低油菜籽的含油量。木质素是针对病原体的既定物理屏障，并且不同的木质素单体G和S含量可以具有不同的木质素结构。在这项研究中，在B中，通过CRISPR/Cas9同时敲除四个显性表达的BnF 5 H基因。napus，并产生了f5 h突变体。基于KO-7系中的M柱染色和2D-NMR谱分析的结果，与野生型（WT）相比，S-/G-木质素组成比降低。对S.茎和叶中的菌核在敲除系中增加。此外，我们发现突变体的茎强度与WT相比显著增加。总的来说，我们第一次证明了木质素途径基因F5 H的敲除降低了S/G比，提高了S。B对菌核病的抗性。增加了茎的强度
Ferulate‐5‐hydroxylase is a key enzyme involved in the conversion of the guaiacyl monolignol to the syringyl monolignol in angiosperms. The monolignol ratio has been proposed to affect biomass recalcitrance and the resistance to plant disease. Stem rot caused by the fungus Sclerotinia sclerotiorum in Brassica napus causes severe losses in its production. To date, there is no information about the effect of the lignin monomer ratio on the resistance to S. sclerotiorum in B. napus. Four dominantly expressed ferulate‐5‐hydroxylase genes were concertedly knocked out by CRISPR/Cas9 in B. napus, and three mutant lines were generated. The S/G lignin compositional ratio was decreased compared to that of the wild type based on the results of Mӓule staining and 2D‐NMR profiling in KO‐7. The resistance to S. sclerotiorum in stems and leaves increased for the three f5h mutant lines compared with WT. Furthermore, we found that the stem strength of f5h mutant lines was significantly increased. Overall, we demonstrate for the first time that decreasing the S/G ratio by knocking out of the F5H gene improves S. sclerotiorum resistance in B. napus and increases stem strength. The oilseed rape production is unremittingly affected by Sclerotinia sclerotiorum, which causes stem and leaf rot and reduces the production of rapeseed oil. Lignin is an established physical barrier against pathogens, and the different lignin monomer G and S content can have different lignin structures. In this study, four dominantly expressed BnF5H genes were knocked out by CRISPR/Cas9 simultaneously in B. napus, and the f5h mutants were generated. The S‐/G‐lignin composition ratio was decreased compared to that of the wild type (WT) based on the results of Mӓule staining and 2D‐NMR profiling in the KO‐7 line. The resistance to S. sclerotiorum in stems and leaves increased in knock‐out lines. Furthermore, we found that the stem strength of mutants was significantly increased compared to that of the WT. Collectively, for the first time, we demonstrate that knockout of the lignin pathway gene F5H decreases the S/G ratio, improves S. sclerotiorum resistance in B. napus, and increases stem strength.
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影响因子：
6.9
作者：
Bhuiyan NH;Selvaraj G;Wei Y;King J
通讯作者：
King J
影响因子：
7.2
作者：
Franke, R;McMichael, CM;Chapple, C
通讯作者：
Chapple, C
影响因子：
3.8
作者：
Donaldson, LA
通讯作者：
Donaldson, LA
影响因子：
3.8
作者：
Ko, Ja Kyong;Ximenes, Eduardo;Ladisch, Michael R.
通讯作者：
Ladisch, Michael R.
影响因子：
6.2
作者：
Cardoza, V;Stewart, CN
通讯作者：
Stewart, CN