Gene expression profiling and silencing reveal that monolignol biosynthesis plays a critical role in penetration defence in wheat against powdery mildew invasion.
Gene expression profiling and silencing reveal that monolignol biosynthesis plays a critical role in penetration defence in wheat against powdery mildew invasion.
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DOI：
10.1093/jxb/ern290
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发表时间：
2009
影响因子：
6.9
通讯作者：
King J
中科院分区：
文献类型：
--
作者：
Bhuiyan NH;Selvaraj G;Wei Y;King J
细胞壁并置（CWA）的形成是植物用来阻止白粉病等真菌入侵的第一道防线之一。木质素是羟基化和甲氧基化苯丙烷单元（木质素单体）的复杂聚合物，木质素化使细胞壁更能抵抗病原体的攻击。本文使用 RNA 干扰 (RNAi) 介导的基因沉默和酶特异性抑制剂证明了木质素单体生物合成在 CWA 介导的防御白粉病渗透到谷物中的作用。从表达序列标签 (EST) 文库中克隆了 13 个 cDNA，代表参与木质素生物合成的 8 个基因，该文库来源于感染了 Blumeria graminis f 的二倍体小麦 (Triticum monocaccum) 的表皮。 sp。小麦（Bgt）。感染后，在易感植物和抗性植物中发现了这些基因的差异表达模式。苯丙氨酸氨裂解酶（PAL）、咖啡酸O-甲基转移酶（CAOMT）、阿魏酸羟化酶（FAH）、咖啡酰辅酶A O-甲基转移酶（CCoAMT）和肉桂醇脱氢酶（CAD）的转录物积累，特别是在表皮中。表皮中 RNAi 介导的瞬时基因沉默导致 Bgt 的渗透效率高于对照组。基因沉默还不同程度地损害了针对不适当病原体（B. graminis f）的不同基因的渗透抗性。 sp。大麦 (Bgh)。与基因单独沉默相比，共同沉默导致 Bgt 或 Bgh 的渗透更大。荧光发射光谱分析表明，基因沉默阻碍了真菌接触位点的宿主自发荧光反应。这些结果表明，木质醇单体生物合成对于宿主防御小麦中适当和不适当的病原体入侵至关重要。
Cell wall apposition (CWA) formation is one of the first lines of defence used by plants to halt invading fungi such as powdery mildew. Lignin is a complex polymer of hydroxylated and methoxylated phenylpropane units (monolignols) and lignification renders the cell wall more resistant to pathogen attack. The role of monolignol biosynthesis in CWA-mediated defence against powdery mildew penetration into cereals is demonstrated here using RNA interference (RNAi)-mediated gene silencing and enzyme-specific inhibitors. Thirteen cDNAs representing eight genes involved in monolignol biosynthesis were cloned from an expression sequence tag (EST) library derived from the epidermis of diploid wheat (Triticum monococcum) infected with Blumeria graminis f. sp. tritici (Bgt). Differential expression patterns were found for these genes in susceptible and resistant plants after infection. Transcripts of phenylalanine ammonia lyase (PAL), caffeic acid O-methyltransferase (CAOMT), ferulic acid hydroxylase (FAH), caffeoyl-CoA O-methyltransferase (CCoAMT), and cinnamyl alcohol dehydrogenase (CAD) were accumulated, particularly in the epidermis. RNAi-mediated transient gene silencing in the epidermis led to a higher penetration efficiency of Bgt than in the controls. Gene silencing also compromised penetration resistance to varying degrees with different genes against an inappropriate pathogen, B. graminis f. sp. hordei (Bgh). Co-silencing led to greater penetration of Bgt or Bgh than when the genes were silenced separately. Fluorescence emission spectra analyses revealed that gene silencing hampered host autofluorescence response at fungal contact sites. These results illustrate that monolignol biosynthesis is critically important for host defence against both appropriate and inappropriate pathogen invasion in wheat.
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影响因子：
2.7
作者：
Kruger, WM;Carver, TLW;Zeyen, RJ
通讯作者：
Zeyen, RJ
影响因子：
4.3
作者：
McInnes, R;Lidgett, A;Spangenberg, G
通讯作者：
Spangenberg, G
影响因子：
7.4
作者：
KAUSS, H;FRANKE, R;MATERN, U
通讯作者：
MATERN, U
影响因子：
7.4
作者：
FRITZEMEIER, KH;CRETIN, C;HAHLBROCK, K
通讯作者：
HAHLBROCK, K
影响因子：
5.1
作者：
Koutaniemi, Sanna;Warinowski, Tino;Teeri, Teemu H.
通讯作者：
Teeri, Teemu H.