Light and electron microscopic studies on the localization of steroid-binding protein (SBP) in rabbit spermatozoa.
Light and electron microscopic studies on the localization of steroid-binding protein (SBP) in rabbit spermatozoa.
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兔精子中类固醇结合蛋白（SBP）定位的光和电子显微镜研究。
DOI：
10.1095/biolreprod33.2.503
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发表时间：
1985
影响因子：
3.6
通讯作者：
Farr,AG
中科院分区：
文献类型：
--
作者：
David,GF;Koehler,JK;Brown,JA;Petra,PH;Farr,AG
用光学(荧光)和电子显微镜研究了类固醇结合蛋白(SBP)在兔精子中的定位。未通透性和通透性(用吐温20、皂苷或冷丙酮)的精子在抗兔SBP(抗RSBP)和随后用兔抗羊免疫球蛋白G-异硫氰酸荧光素处理后都显示出荧光。当射出的精子呈阳性时，附睾精子呈阴性。尽管RSBP的定位模式是可变的，但在头部和中段交界处出现的负赤道区以及强烈的正球形轮廓(“斑点”)是显著一致的。在冷丙酮渗透后，该探针在光镜和电子显微镜水平上的标记强度最大。SBP可能作为类固醇载体蛋白穿过精子的质膜。
Light (fluorescence) and electron microscopic studies were carried out to localize steroid-binding protein (SBP) in rabbit spermatozoa. Both nonpermeabilized and permeabilized (with Tween 20, saponin, or cold acetone) spermatozoa showed fluorescence following treatment with antirabbit SBP (anti-rSBP) and subsequently with rabbit antisheep immunoglobulin G-fluorescein isothiocyanate. While the ejaculated spermatozoa were positive, epididymal sperm were observed to be negative. Although the pattern of localization of rSBP was variable, the occurrence of a negative equatorial region as well as the presence of an intense positive spherical profile (“spot”) at the junction of the head and midpiece were notably consistent.The intensity of labeling with the probe, both at light and electron microscopic level, was maximal following permeabilization with cold acetone.A possible role of SBP as a steroid carrier protein across the plasma membrane of the sperm has been suggested.
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通过亲和层析纯化人血清性类固醇结合蛋白的改进。
影响因子：
2.9
作者：
P. Pétra;J. Lewis
通讯作者：
J. Lewis
在“获能”培养基中孵育后，兔精子上抗原位点分布的变化。
影响因子：
3.6
作者：
J. Koehler
通讯作者：
J. Koehler
附睾、射精和获能精子的表面涂层。
DOI：
10.1016/s0022-5320(75)80051-7
发表时间：
1975
期刊：
Journal of ultrastructure research
影响因子：
--
作者：
M. Gordon;P. Dandekar;W. Bartoszewicz
通讯作者：
W. Bartoszewicz
抗体标记技术
DOI：
10.1007/978-3-642-66809-8_2
发表时间：
1978
期刊：
Journal of ultrastructure research
影响因子：
--
作者：
W. Perkins;J. Koehler
通讯作者：
J. Koehler
猕猴血清中性类固醇结合蛋白的纯化和表征。
影响因子：
2.9
作者：
Turner,EE;Ross,JB;Namkung,PC;Petra,PH
通讯作者：
Petra,PH