CRE/LOX-based analysis of cell lineage during root formation and regeneration in Arabidopsis

CRE/LOX-based analysis of cell lineage during root formation and regeneration in Arabidopsis
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基于 CRE/LOX 的拟南芥根形成和再生过程中的细胞谱系分析

DOI:
10.1007/s42994-020-00025-y
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发表时间:
2020-07
期刊:
aBIOTECH
影响因子:
--
通讯作者:
Xu Lin
Xu Lin
中科院分区:
其他
文献类型:
--
作者:
Zhai Ning;Xu Lin

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拟南芥的根系包括原生根、侧根和不定根。不同类型的根是由不同的诱导线索和发育程序形成的。在此,我们采用CRE/LOX系统在wuschell相关的HOMEOBOX5启动子的控制下,对三种根形成过程中的细胞谱系进行了追踪。结果表明,离体叶片和主根的侧根再生过程中形成不定根的细胞是表达wox5的根原基的后代。在初代根胚后生长过程中,一些维管细胞和根冠细胞是根尖分生组织中表达wox5的干细胞生态位的后代。总的来说,我们的数据表明,CRE/LOX系统是追踪不同类型根器官发生的细胞谱系的有用工具。
The root system of Arabidopsis thaliana comprises primary, lateral, and adventitious roots. Different types of roots are formed by diverse inductive cues and developmental programs. Here, we adopted the CRE/LOX system to trace cell lineage during the three types of root formation under the control of the promoter of WUSCHEL-RELATED HOMEOBOX5. The results show that the cells forming adventitious roots during de novo root regeneration from detached leaves and lateral roots from the primary root are descendants of the WOX5-expressing root primordium. During the post-embryonic growth of the primary root, some vascular and root cap cells are descendants of the WOX5-expressing stem cell niche in the root apical meristem. Overall, our data suggest that the CRE/LOX system is a useful tool to trace cell lineage in different types of root organogenesis.
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