MALDI-2 for the Enhanced Analysis of N-Linked Glycans by Mass Spectrometry Imaging.
MALDI-2 for the Enhanced Analysis of N-Linked Glycans by Mass Spectrometry Imaging.
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DOI：
10.1021/acs.analchem.0c02732
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发表时间：
2020-10-20
影响因子：
7.4
通讯作者：
Dreisewerd K
中科院分区：
文献类型：
--
作者：
Heijs B;Potthoff A;Soltwisch J;Dreisewerd K
N-聚糖是多种病理学的重要参与者，包括不同类型的癌症、（自身）免疫疾病以及病毒感染。基质辅助激光解吸/电离质谱（MALDI-MS）是高通量N-聚糖分析的重要工具，在使用串联MS时，用于结构测定。通过使用MALDI-MS成像（MSI）与PNGase F处理组合，来自组织切片的空间相关N-聚糖谱分析也成为可能。在这里，我们耦合激光诱导定位，或MALDI-2，捕获离子迁移率四极杆飞行时间质谱仪（timsTOF fleX MALDI-2，布鲁克道尔顿）。我们证明，使用MALDI-2，检测N-聚糖的分子[M-H]−种类的灵敏度增加了约3个数量级。与目前的金标准相比，[M + Na]+加合物的正离子模式分析的灵敏度提高了约10倍。此外，通过利用[M-H]−离子的有利的碎裂行为，直接从人小脑的薄组织切片和使用低能碰撞诱导解离串联MS获得了关于复合N-聚糖组成的极其丰富的结构信息。在另一组实验中，在这种情况下，通过使用改进的Synapt G2-S QTOF质谱仪（沃茨），我们研究了相关输入参数的影响，特别是离子源中N2冷却气体的压力、两个激光脉冲之间的延迟以及它们的脉冲能量。以这种方式，确定了分子离子丰度最大化和碎裂反应最小化的分析条件。负离子模式MALDI-2-MSI的使用可构成糖生物学研究中的有价值的工具。
N-glycans are important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination. By use of MALDI-MS imaging (MSI) in combination with PNGase F treatment, also spatially correlated N-glycan profiling from tissue sections becomes possible. Here we coupled laser-induced postionization, or MALDI-2, to a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics). We demonstrate that with MALDI-2 the sensitivity for the detection of molecular [M – H]− species of N-glycans increased by about 3 orders of magnitude. Compared to the current gold standard, the positive ion mode analysis of [M + Na]+ adducts, a sensitivity increase by about a factor of 10 is achieved. By exploiting the advantageous fragmentation behavior of [M – H]− ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies. In this way, analytical conditions were identified at which molecular ion abundances were maximized and fragmentation reactions minimized. The use of negative ion mode MALDI-2-MSI could constitute a valuable tool in glycobiology research.
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影响因子：
4.6
作者：
Niehaus M;Soltwisch J
通讯作者：
Soltwisch J
激光诱导解离负离子 MALDI 串联质谱法对中性寡糖的连接和序列分析
影响因子：
6.2
作者：
Huang，Chuncui;Yan，Jingyu;Chai，Wengang
通讯作者：
Chai，Wengang
影响因子：
7.4
作者：
Boskamp, Marcel S.;Soltwisch, Jens
通讯作者：
Soltwisch, Jens
DOI：
10.1016/j.jasms.2005.01.005
发表时间：
2005-05-01
影响因子：
3.2
作者：
Harvey, DJ
通讯作者：
Harvey, DJ
影响因子：
3.4
作者：
Briggs, Matthew T.;Condina, Mark R.;Hoffmann, Peter
通讯作者：
Hoffmann, Peter