The performance of cross-linked acellular arterial scaffolds as vascular grafts; pre-clinical testing in direct and isolation loop circulatory models.
The performance of cross-linked acellular arterial scaffolds as vascular grafts; pre-clinical testing in direct and isolation loop circulatory models.
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DOI：
10.1016/j.biomaterials.2014.04.062
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发表时间：
2014-08
期刊：
影响因子：
14
通讯作者：
Simionescu, Dan
中科院分区：
文献类型：
--
作者：
Pennel, Timothy;Fercana, George;Bezuidenhout, Deon;Simionescu, Agneta;Chuang, Ting-Hsien;Zilla, Peter;Simionescu, Dan
外周血管手术中非常需要使用小直径血管移植物;然而，自体移植物并不总是可用的，合成移植物表现不佳，同种异体移植物和异种移植物在植入后退化、扩张和钙化。我们假设，化学稳定脱细胞异种动脉将产生现成的移植物抗血栓形成，扩张和钙化。为了验证这一假设，我们脱细胞猪肾动脉，稳定弹性蛋白与五没食子酰葡萄糖和胶原与碳二亚胺/活化肝素，并将它们作为转位移植物植入大鼠腹主动脉作为直接植入物和分别作为间接，隔离环植入物。所有植入物均具有较高的通畅率和动物存活率，血管化胶原囊内普遍存在包囊，并且表现出缺乏管腔血栓形成性和无移植物壁钙化。吻合口周围新生内膜组织过度生长在直接植入中是正常发生的;然而，在间接植入中避免了这种反应。值得注意的是，未处理的对照支架的植入表现出明显的移植物扩张和弹性蛋白变性;然而，PGG显著减少弹性蛋白降解并防止血管移植物的血管扩张。总的来说，这些结果指出了交联动脉支架作为小直径血管移植物的突出潜力。
There is a significant need for small diameter vascular grafts to be used in peripheral vascular surgery; however autologous grafts are not always available, synthetic grafts perform poorly and allografts and xenografts degenerate, dilate and calcify after implantation. We hypothesized that chemical stabilization of acellular xenogenic arteries would generate off-the-shelf grafts resistant to thrombosis, dilatation and calcification. To test this hypothesis, we decellularized porcine renal arteries, stabilized elastin with penta-galloyl glucose and collagen with carbodiimide / activated heparin and implanted them as transposition grafts in the abdominal aorta of rats as direct implants and separately as indirect, isolation-loop implants. All implants resulted in high patency and animal survival rates, ubiquitous encapsulation within a vascularized collagenous capsule, and exhibited lack of lumen thrombogenicity and no graft wall calcification. Peri-anastomotic neo-intimal tissue overgrowth was a normal occurrence in direct implants; however this reaction was circumvented in indirect implants. Notably, implantation of non-treated control scaffolds exhibited marked graft dilatation and elastin degeneration; however PGG significantly reduced elastin degradation and prevented aneurismal dilatation of vascular grafts. Overall these results point to the outstanding potential of crosslinked arterial scaffolds as small diameter vascular grafts.
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影响因子：
4.3
作者：
Tai, NRM;Giudiceandrea, A;Hamilton, G
通讯作者：
Hamilton, G
影响因子：
37.8
作者：
Isenburg, Jason C.;Simionescu, Dan T.;Vyavahare, Narendra R.
通讯作者：
Vyavahare, Narendra R.
影响因子：
2.7
作者：
Naito, Yuji;Williams-Fritze, Misty;Breuer, Christopher K.
通讯作者：
Breuer, Christopher K.
影响因子：
14
作者：
Chow, James P.;Simionescu, Dan T.;Simionescu, Agneta
通讯作者：
Simionescu, Agneta
影响因子：
4.1
作者：
Mercuri, Jeremy J.;Patnaik, Sourav;Simionescu, Dan T.
通讯作者：
Simionescu, Dan T.