Developmental stage-specific imprinting of IPL in domestic pigs (Sus scrofa).
Developmental stage-specific imprinting of IPL in domestic pigs (Sus scrofa).
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DOI：
10.1155/2010/527539
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发表时间：
2010
影响因子：
--
通讯作者：
Zhang S
中科院分区：
文献类型：
--
作者：
Hou S;Chen Y;Liang J;Li L;Wu T;Tian XC;Zhang S
胎盘和肝脏印记基因（Imprinted in placenta and liver，IPL）是一种在小鼠和人类中发现的印记基因。然而，其序列和印迹状态尚未在家猪中确定。本研究获得了家猪IPL基因259个碱基对的特异性序列，并在IPL外显子1中发现了一个新的SNP，即T/C转换。在长白猪、约克郡猪和杜洛克猪中，C等位基因占优势。杜洛克猪与约克郡猪相比，CC基因型和C等位基因频率差异显著（P = 0.038和P = 0.005）。该基因在不同发育阶段的印记状态不同。例如，它在1天大的新生儿中有印记（从母体等位基因表达），但在180天大的成年人中失去印记（从两个亲本等位基因表达）。Real-time PCR分析表明，猪IPL基因在所有检测的8种器官/组织中均有表达。180日龄成年蓝塘猪脾脏、十二指肠、肺和膀胱中的表达水平显著高于1日龄新生蓝塘猪（P < .05）。因此，猪IPL基因的印迹具有发育阶段和组织特异性。
Imprinted in placenta and liver (IPL) gene has been identified as an imprinted gene in the mouse and human. Its sequence and imprinting status, however, have not been determined in the domestic pigs. In the present study, a 259 base pair-specific sequence for IPL gene of the domestic pig was obtained and a novel SNP, a T/C transition, was identified in IPL exon 1. The C allele of this polymorphism was found to be the predominant allele in Landrace,Yorkshire, and Duroc. The frequency of CC genotype and C allele are different in Duroc as compared with Yorkshire (P = .038 and P = .005, resp.). Variable imprinting status of this gene was observed in different developmental stages. For example, it is imprinted in 1-dayold newborns (expressed from the maternal allele), but imprinting was lost in 180-day-old adult (expressed from both parental alleles). Real-time PCR analysis showed the porcine IPL gene is expressed in all tested eight organ/tissues. The expression level was significantly higher in spleen, duodenum, lung, and bladder of 180-day-old Lantang adult compared to that in 1-day-old newborns Lantang pigs (P < .05). In conclusion, the imprinting of the porcine IPL gene is developmental stage and tissue specific.
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影响因子：
3.5
作者：
M端ller, S;van den Boom, D;Zumkeller, W
通讯作者：
Zumkeller, W
DOI：
10.1073/pnas.86.8.2766
发表时间：
1989-04-01
影响因子：
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作者：
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作者：
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作者：
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通讯作者：
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DOI：
10.1073/pnas.0334222100
发表时间：
2003-02-04
影响因子：
11.1
作者：
Springer, MS;Murphy, WJ;O'Brien, SJ
通讯作者：
O'Brien, SJ