Organoids as host models for infection biology - a review of methods.
Organoids as host models for infection biology - a review of methods.
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DOI：
10.1038/s12276-021-00629-4
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发表时间：
2021-10
影响因子：
12.8
通讯作者：
Bartfeld S
中科院分区：
文献类型：
--
作者：
Aguilar C;Alves da Silva M;Saraiva M;Neyazi M;Olsson IAS;Bartfeld S
传染病是世界范围内的一大威胁。随着抗生素耐药性的惊人上升和新的潜在病原体的出现，迫切需要更好地了解感染过程。在上个世纪，体外和体内模型的发展对感染生物学领域的现有知识做出了显著贡献。然而，将类器官培养技术的最新进展应用于研究传染病，现在正在将该领域推向更高的复杂程度。在这里，我们描述了目前的方法可用于研究传染病的类器官培养。使用微型化的三维器官和组织来模拟传染病可以提高对患者特异性反应的理解，并为个性化治疗提供信息。类器官培养物由组织特异性成体干细胞产生，并重建给定器官或组织的一些原始细胞结构。它们还模仿器官的分子机制和反应。德国维尔茨堡Julius Maximilians大学的Sina Bartfeld及其同事回顾了最近对类器官培养用于感染和疾病进展建模的研究。从不同的个体中创造类器官使科学家能够监测感染在独特宿主中的行为。类器官研究强调了细菌初始感染的特定目标，确定了影响疾病结果的宿主因素，并阐明了患者对治疗的特异性反应。未来的类器官应该更加复杂，包括免疫细胞和神经细胞，甚至包括微生物组。
Infectious diseases are a major threat worldwide. With the alarming rise of antimicrobial resistance and emergence of new potential pathogens, a better understanding of the infection process is urgently needed. Over the last century, the development of in vitro and in vivo models has led to remarkable contributions to the current knowledge in the field of infection biology. However, applying recent advances in organoid culture technology to research infectious diseases is now taking the field to a higher level of complexity. Here, we describe the current methods available for the study of infectious diseases using organoid cultures. Using miniaturized, three-dimensional versions of organs and tissues to model infectious diseases could improve understanding of patient-specific responses and inform personalized therapies. Organoid cultures are generated from tissue-specific adult stem cells, and recreate some of the original cellular structure of a given organ or tissue. They also mimic the organ’s molecular mechanisms and responses. Sina Bartfeld at the Julius Maximilians Universität Würzburg, Germany, and coworkers reviewed recent research into organoid cultures for modeling infections and disease progression. Creating organoids from different individuals allows scientists to monitor how infections behave in unique hosts. Organoid studies have highlighted specific targets for initial infection by bacteria, identified host factors that influence disease outcomes, and clarified patient-specific responses to treatments. Future organoids should be more complex, incorporating immune and nerve cells, and even factoring in the microbiome.
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影响因子：
30.3
作者：
Burger E;Araujo A;López-Yglesias A;Rajala MW;Geng L;Levine B;Hooper LV;Burstein E;Yarovinsky F
通讯作者：
Yarovinsky F
DOI：
10.1073/pnas.1811866115
发表时间：
2018-10-02
影响因子：
11.1
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影响因子：
1.2
作者：
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通讯作者：
O'Connor, Roberta
影响因子：
30.3
作者：
Grassart, Alexandre;Malarde, Valerie;Sauvonnet, Nathalie
通讯作者：
Sauvonnet, Nathalie
影响因子：
24.5
作者：
Boccellato F;Woelffling S;Imai-Matsushima A;Sanchez G;Goosmann C;Schmid M;Berger H;Morey P;Denecke C;Ordemann J;Meyer TF
通讯作者：
Meyer TF