Coupled induction of prophage and virulence factors during tick transmission of the Lyme disease spirochete.
Coupled induction of prophage and virulence factors during tick transmission of the Lyme disease spirochete.
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DOI：
10.1038/s41467-023-35897-3
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发表时间：
2023-01-13
影响因子：
16.6
通讯作者：
Rosa, Patricia A.
中科院分区：
文献类型：
--
作者：
Wachter, Jenny;Cheff, Britney;Hillman, Chad;Carracoi, Valentina;Dorward, David W.;Martens, Craig;Barbian, Kent;Nardone, Glenn;Olano, L. Renee;Kinnersley, Margie;Secor, Patrick R.;Rosa, Patricia A.
The alternative sigma factor RpoS plays a central role in the critical host-adaptive response of the Lyme disease spirochete, Borrelia burgdorferi. We previously identified bbd18 as a negative regulator of RpoS but could not inactivate bbd18 in wild-type spirochetes. In the current study we employed an inducible bbd18 gene to demonstrate the essential nature of BBD18 for viability of wild-type spirochetes in vitro and at a unique point in vivo. Transcriptomic analyses of BBD18-depleted cells demonstrated global induction of RpoS-dependent genes prior to lysis, with the absolute requirement for BBD18, both in vitro and in vivo, circumvented by deletion of rpoS. The increased expression of plasmid prophage genes and the presence of phage particles in the supernatants of lysing cultures indicate that RpoS regulates phage lysis-lysogeny decisions. Through this work we identify a mechanistic link between endogenous prophages and the RpoS-dependent adaptive response of the Lyme disease spirochete. The alternative sigma factor RpoS of Borrelia burgdorferi regulates a pathway implicated in tick transmission, mammalian infectivity and persistent infection. Here, the authors characterise the role of the RpoS negative regulator bbd18 and show that RpoS also controls induction of endogenous prophage.
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影响因子：
3.7
作者：
Chen L;Xu Q;Tu J;Ge Y;Liu J;Liang FT
通讯作者：
Liang FT
影响因子：
3.1
作者：
Barthold, SW
通讯作者：
Barthold, SW
影响因子：
3.2
作者：
Caimano, MJ;Eggers, CH;Radolf, JD
通讯作者：
Radolf, JD
DOI：
10.1073/pnas.1718595115
发表时间：
2018-04-17
影响因子：
11.1
作者：
Bernard, Quentin;Smith, Alexis A.;Pal, Utpal
通讯作者：
Pal, Utpal
影响因子：
3.2
作者：
Casjens, S;vanVugt, R;Stevenson, B
通讯作者：
Stevenson, B