Mechanical wounding induces a nitrosative stress by down-regulation of GSNO reductase and an increase in S-nitrosothiols in sunflower (Helianthus annuus) seedlings.
Mechanical wounding induces a nitrosative stress by down-regulation of GSNO reductase and an increase in S-nitrosothiols in sunflower (Helianthus annuus) seedlings.
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DOI：
10.1093/jxb/erq358
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发表时间：
2011-03
影响因子：
6.9
通讯作者：
Barroso JB
中科院分区：
文献类型：
--
作者：
Chaki M;Valderrama R;Fernández-Ocaña AM;Carreras A;Gómez-Rodríguez MV;Pedrajas JR;Begara-Morales JC;Sánchez-Calvo B;Luque F;Leterrier M;Corpas FJ;Barroso JB
一氧化氮 (NO) 和相关分子，例如过氧亚硝酸盐、S-亚硝基谷胱甘肽 (GSNO) 和硝基酪氨酸等，参与生理过程以及对应激条件的反应机制。暴露于五种不同不利环境条件（低温、机械伤、强光、持续光照、持续黑暗）的向日葵幼苗中，活性氮（RNS）和活性氧（ROS）代谢的关键成分，包括L-精氨酸依赖性一氧化氮合酶（NOS）、S-亚硝基谷胱甘肽还原酶的酶活性 分析了硝酸还原酶（GSNOR）、硝酸还原酶（NR）、过氧化氢酶和超氧化物歧化酶，脂质过氧化氢、过氧化氢、S-亚硝基硫醇（SNOs）的含量，NO、GSNO和GSNOR的细胞水平以及蛋白质酪氨酸硝化[硝基酪氨酸（NO2-Tyr）]。在所研究的应激条件中，机械损伤是唯一一种导致 NOS 和 GSNOR 活性下调的应激条件，进而引发 SNO 的积累。通过共焦激光扫描显微镜对 NO、GSNO、GSNOR 和 NO2-Tyr 的细胞含量进行的分析证实了这些生化数据。因此，有人提出，由于 GSNOR 活性下调，机械损伤会触发 SNO，特别是 GSNO 的积累，同时 NO2-Tyr 增加。因此，向日葵幼苗中会引发亚硝化胁迫过程，SNO 会在植物中构成新的伤口信号。
Nitric oxide (NO) and related molecules such as peroxynitrite, S-nitrosoglutathione (GSNO), and nitrotyrosine, among others, are involved in physiological processes as well in the mechanisms of response to stress conditions. In sunflower seedlings exposed to five different adverse environmental conditions (low temperature, mechanical wounding, high light intensity, continuous light, and continuous darkness), key components of the metabolism of reactive nitrogen species (RNS) and reactive oxygen species (ROS), including the enzyme activities L-arginine-dependent nitric oxide synthase (NOS), S-nitrosogluthathione reductase (GSNOR), nitrate reductase (NR), catalase, and superoxide dismutase, the content of lipid hydroperoxide, hydrogen peroxide, S-nitrosothiols (SNOs), the cellular level of NO, GSNO, and GSNOR, and protein tyrosine nitration [nitrotyrosine (NO2-Tyr)] were analysed. Among the stress conditions studied, mechanical wounding was the only one that caused a down-regulation of NOS and GSNOR activities, which in turn provoked an accumulation of SNOs. The analyses of the cellular content of NO, GSNO, GSNOR, and NO2-Tyr by confocal laser scanning microscopy confirmed these biochemical data. Therefore, it is proposed that mechanical wounding triggers the accumulation of SNOs, specifically GSNO, due to a down-regulation of GSNOR activity, while NO2-Tyr increases. Consequently a process of nitrosative stress is induced in sunflower seedlings and SNOs constitute a new wound signal in plants.
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影响因子：
56.9
作者：
BOYER, JS
通讯作者：
BOYER, JS
影响因子：
9.4
作者：
Corpas, FJ;Sandalio, LM;Trelease, RN
通讯作者：
Trelease, RN
影响因子：
11.6
作者：
Creissen, G;Firmin, J;Mullineaux, P
通讯作者：
Mullineaux, P
影响因子：
7.2
作者：
Chang, CCC;Ball, L;Mullineaux, PM
通讯作者：
Mullineaux, PM
影响因子：
4.9
作者：
Corpas, Francisco J.;Chaki, Mounira;Barroso, Juan B.
通讯作者：
Barroso, Juan B.