Long distance PELDOR measurements on the histone core particle.
Long distance PELDOR measurements on the histone core particle.
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DOI：
10.1021/ja807918f
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发表时间：
2009-02-04
影响因子：
15
通讯作者：
Norman DG
中科院分区：
文献类型：
--
作者：
Ward R;Bowman A;El-Mkami H;Owen-Hughes T;Norman DG
核小体核心粒子是染色质结构的基本单位，其核心是由组蛋白H4、H3、H2A和H2B组成的组蛋白核心八聚体。为了了解染色质的结构动力学和功能，能够以各种方式探测其组成部分的结构是很重要的。位点定向自旋标记技术可以在H3组蛋白表面的位置插入氮氧化物自旋标记，并将这些自旋标记组装成组蛋白八聚体。脉冲EPR，特别是PELDOR或DEER实验已经进行，并提供了非常明确的偶极振荡，在很长一段时间内。从PELDOR的数据中，我们已经能够得出60到70之间的距离分布Å。测量的距离是迄今为止在生物系统上定义的最长的PELDOR测量，跨越组蛋白核心颗粒的宽度，并接近该技术通常定义的距离测量极限。在晶体结构上已经观察到相对较小的差异。
The nucleosome core particle is the fundamental unit of chromatin structure and at its heart is the histone core octamer composed of histones H4, H3, H2A and H2B. To understand the structure dynamics and function of chromatin it is important to be able to probe the structures of its component parts in a variety of ways. Site directed spin-labeling technology has enabled the insertion of nitroxide spin labels at positions on the surface of the H3 histones and these have been assembled into histone octamers. Pulsed EPR, and in particular the PELDOR or DEER experiments have been performed and provided extremely well defined dipolar oscillations, over long time periods. From the PELDOR data we have been able to derive distance distributions of between 60 and 70 Å. The distances measured, are among the longest well-defined PELDOR measurements on a biological system to date, spanning the width of the histone core particle and approaching what has been often defined as the limit of distance measurement by this technique. Relatively minor differences to the crystal structures have been observed.
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