Microwaves from Mobile Phones Inhibit 53BP1 Focus Formation in Human Stem Cells More Strongly Than in Differentiated Cells: Possible Mechanistic Link to Cancer Risk.
Microwaves from Mobile Phones Inhibit 53BP1 Focus Formation in Human Stem Cells More Strongly Than in Differentiated Cells: Possible Mechanistic Link to Cancer Risk.
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DOI：
10.1289/ehp.0900781
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发表时间：
2010-03
影响因子：
10.4
通讯作者：
Belyaev IY
中科院分区：
文献类型：
--
作者：
Markovà E;Malmgren LO;Belyaev IY
人们普遍认为，干细胞中的DNA双链断裂（DSB）及其误修复是各种白血病和肿瘤（包括神经胶质瘤）多阶段起源的关键事件。我们研究了来自全球移动的通信系统（GSM）和通用全球电信系统（UMTS）的移动的电话的微波是否诱导干细胞中的DSB或影响DSB修复。我们分析了肿瘤抑制因子TP53结合蛋白1（53BP1）的焦点，通常形成在DSB位置的网站（称为DNA修复灶），通过激光共聚焦显微镜。来自移动的手机的微波抑制人原代成纤维细胞和间充质干细胞中53 BP 1灶的形成。这些数据与我们先前对人类淋巴细胞的发现相一致。重要的是，相同的GSM载波频率（915 MHz）和UMTS频段（1947.4 MHz）对所有类型的小区都有效。暴露在905 MHz并没有抑制53 BP1灶在分化的细胞，无论是成纤维细胞或淋巴细胞，而在干细胞在905 MHz的一些影响。与成纤维细胞相反，干细胞在2周内不适应慢性暴露。在干细胞中总是观察到最强的微波效应。这一结果可能表明DSB修复中的显著失衡和严重的应激反应。我们的研究发现，干细胞是最敏感的微波照射和反应更多的频率比分化细胞可能是重要的癌症风险评估，并表明干细胞是最相关的细胞模型验证安全的移动的通信信号。
It is widely accepted that DNA double-strand breaks (DSBs) and their misrepair in stem cells are critical events in the multistage origination of various leukemias and tumors, including gliomas. We studied whether microwaves from mobile telephones of the Global System for Mobile Communication (GSM) and the Universal Global Telecommunications System (UMTS) induce DSBs or affect DSB repair in stem cells. We analyzed tumor suppressor TP53 binding protein 1 (53BP1) foci that are typically formed at the sites of DSB location (referred to as DNA repair foci) by laser confocal microscopy. Microwaves from mobile phones inhibited formation of 53BP1 foci in human primary fibroblasts and mesenchymal stem cells. These data parallel our previous findings for human lymphocytes. Importantly, the same GSM carrier frequency (915 MHz) and UMTS frequency band (1947.4 MHz) were effective for all cell types. Exposure at 905 MHz did not inhibit 53BP1 foci in differentiated cells, either fibroblasts or lymphocytes, whereas some effects were seen in stem cells at 905 MHz. Contrary to fibroblasts, stem cells did not adapt to chronic exposure during 2 weeks. The strongest microwave effects were always observed in stem cells. This result may suggest both significant misbalance in DSB repair and severe stress response. Our findings that stem cells are most sensitive to microwave exposure and react to more frequencies than do differentiated cells may be important for cancer risk assessment and indicate that stem cells are the most relevant cellular model for validating safe mobile communication signals.
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影响因子：
56.9
作者：
Celeste, A;Petersen, S;Nussenzweig, A
通讯作者：
Nussenzweig, A
影响因子：
1.9
作者：
Belyaev, IY;Hillert, L;Harms-Ringdahl, M
通讯作者：
Harms-Ringdahl, M
影响因子：
64.8
作者：
Brons, I. Gabrielle M.;Smithers, Lucy E.;Vallier, Ludovic
通讯作者：
Vallier, Ludovic
影响因子：
1.9
作者：
d'Ambrosio, G;Massa, R;Zeni, O
通讯作者：
Zeni, O
影响因子：
3.8
作者：
Banuelos, C. A.;Banath, J. P.;Olive, P. L.
通讯作者：
Olive, P. L.