Generic protease detection technology for monitoring periodontal disease.
Generic protease detection technology for monitoring periodontal disease.
复制标题
用于监测牙周病的通用蛋白酶检测技术。
作者：
Xinwei Zheng;Joseph P. Cook;M. Watkinson;Shoufeng Yang;Ian Douglas;A. Rawlinson;S. Krause
牙周病是一种影响牙齿支持组织的炎症性疾病，如果不及时治疗，可能会导致骨支持破坏，最终导致牙齿脱落。牙周炎的进展通常是部位特异性的，但并不统一，目前还没有准确的临床方法来区分活动性疾病进展部位和静止部位。因此，可能会对未进展的牙周部位进行不必要且昂贵的治疗。三种蛋白酶已被确定为区分活动性疾病进展位点和静止位点的合适标记：人中性粒细胞弹性蛋白酶、组织蛋白酶 G 和 MMP8。用于检测这三种蛋白酶的通用传感器材料是基于与肽交联的葡聚糖水凝胶薄膜而开发的。使用阻抗测量来监测水凝胶膜的降解。在 3 分钟的时间内检测到临床相关范围内的目标蛋白酶。通过选择合适的肽交联剂，可以实现对不同蛋白酶的良好特异性。
Periodontal diseases are inflammatory conditions that affect the supporting tissues of teeth and can lead to destruction of the bone support and ultimately tooth loss if untreated. Progression of periodontitis is usually site specific but not uniform, and currently there are no accurate clinical methods for distinguishing sites where there is active disease progression from sites that are quiescent. Consequently, unnecessary and costly treatment of periodontal sites that are not progressing may occur. Three proteases have been identified as suitable markers for distinguishing sites with active disease progression and quiescent sites: human neutrophil elastase, cathepsin G and MMP8. Generic sensor materials for the detection of these three proteases have been developed based on thin dextran hydrogel films cross-linked with peptides. Degradation of the hydrogel films was monitored using impedance measurements. The target proteases were detected in the clinically relevant range within a time frame of 3 min. Good specificity for different proteases was achieved by choosing appropriate peptide cross-linkers.
通过二肽增强的胰蛋白酶样活性检测牙龈渗出液中的牙龈卟啉单胞菌。
影响因子：
4.3
作者：
Wikstrom,M;Potempa,J;Polanowski,A;Travis,J;Renvert,S
通讯作者：
Renvert,S
人成纤维细胞和中性粒细胞胶原酶的序列特异性。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Netzel-Arnett,S;Fields,GB;Birkedal-Hansen,H;VanWart,HE;Fields,G
通讯作者：
Fields,G
牙周病治疗中蛋白酶活性与临床参数之间的关系。
影响因子：
6.7
作者：
Mailhot,JM;Potempa,J;Stein,SH;Travis,J;Sterrett,JD;Hanes,PJ;Russell,CM
通讯作者：
Russell,CM