Meniscus maturation in the swine model: changes occurring along with anterior to posterior and medial to lateral aspect during growth.
Meniscus maturation in the swine model: changes occurring along with anterior to posterior and medial to lateral aspect during growth.
复制标题
DOI：
10.1111/jcmm.12367
复制
发表时间：
2014-10
影响因子：
5.3
通讯作者：
Peretti GM
中科院分区：
文献类型：
--
作者：
Di Giancamillo A;Deponti D;Addis A;Domeneghini C;Peretti GM
半月板在膝关节功能和力学中发挥着重要作用，其特点是基质成分不均匀。在生长过程中观察到的半月板血管化的变化表明组织特异性成分可能是成熟过程的结果。本研究的目的是表征猪半月板随年龄变化而发生的结构和生化变化。为此，从幼猪和成年猪身上收集半月板并分为不同的区域。在研究1中，将外侧半月板和内侧半月板分为前角、体部和后角，以评估糖胺聚糖（GAG）、胶原蛋白1和胶原蛋白2的含量。在研究2中，将半月板分为内区、中间区和外区，通过基因表达分析确定细胞表型沿内外方向的变化。根据结果，猪半月板的特点是随着年龄的增长，软骨成分不断富集，前角沉积不断增加（GAG 和胶原蛋白 2；均 P < 0.01）；此外，由于半月板细胞向软骨表型的特定分化（胶原蛋白 2，P < 0.01），这种软骨基质在内部无血管和中间区强烈增加。获得的数据增加了半月板成熟过程中变化的新信息，表明半月板细胞对膝关节区域机械刺激的特定反应。
The meniscus plays important roles in knee function and mechanics and is characterized by a heterogeneous matrix composition. The changes in meniscus vascularization observed during growth suggest that the tissue-specific composition may be the result of a maturation process. This study has the aim to characterize the structural and biochemical variations that occur in the swine meniscus with age. To this purpose, menisci were collected from young and adult pigs and divided into different zones. In study 1, both lateral and medial menisci were divided into the anterior horn, the body and the posterior horn for the evaluation of glycosaminoglycans (GAGs), collagen 1 and 2 content. In study 2, the menisci were sectioned into the inner, the intermediate and the outer zones to determine the variations in the cell phenotype along with the inner–outer direction, through gene expression analysis. According to the results, the swine meniscus is characterized by an increasing enrichment in the cartilaginous component with age, with an increasing deposition in the anterior horn (GAGs and collagen 2; P < 0.01 both); moreover, this cartilaginous matrix strongly increases in the inner avascular and intermediate zone, as a consequence of a specific differentiation of meniscal cells towards a cartilaginous phenotype (collagen 2, P < 0.01). The obtained data add new information on the changes that accompany meniscus maturation, suggesting a specific response of meniscal cells to the regional mechanical stimuli in the knee joint.
登录
查看更多内容
影响因子：
2.8
作者：
PROCTOR, CS;SCHMIDT, MB;MOW, VC
通讯作者：
MOW, VC
影响因子：
4.1
作者：
Ionescu, Lara C.;Lee, Gregory C.;Mauck, Robert L.
通讯作者：
Mauck, Robert L.
影响因子：
2.2
作者：
Petersen, W;Pufe, T;Tillmann, B
通讯作者：
Tillmann, B
影响因子：
2.9
作者：
CHEUNG, HS
通讯作者：
CHEUNG, HS
影响因子：
6.9
作者：
Pufe, T;Petersen, WJ;Tillmann, BN
通讯作者：
Tillmann, BN