Interleukin-17A and interleukin-23 in morphea.
Interleukin-17A and interleukin-23 in morphea.
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DOI：
10.5114/aoms.2012.32421
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发表时间：
2012-12-20
期刊：
影响因子：
--
通讯作者：
Hrycaj P
中科院分区：
文献类型：
--
作者：
Dańczak-Pazdrowska A;Kowalczyk M;Szramka-Pawlak B;Gornowicz-Porowska J;Szewczyk A;Silny W;Olewicz-Gawlik A;Molińska-Glura M;Zaba R;Hrycaj P
Morphea是一种硬皮病型自身免疫性疾病。白细胞介素(IL)-17A可能在其发病的各个阶段发挥作用。本研究旨在评价IL-17A和IL-23（作为刺激和维持IL-17合成的主要细胞因子）在morphea发病机制中的作用。这些研究是在41个吗啡患者的血液样本上进行的。29例患者皮肤取样。评估方法包括：(1)采用实时聚合酶链反应（real-time polymerase chain reaction， PCR）检测外周血单个核细胞（PBMC）中IL-17A和IL-23基因的表达；(2)采用ELISA检测IL-17A和IL-23的血浆浓度；(3)采用实时PCR检测皮肤中IL-17A和IL-23基因的表达。基因表达结果以GAPDH每百万拷贝的中位数拷贝数表示。与对照组相比，morphea患者PBMC中IL-17A的表达较高（分别为2630和1906，p = 0.004），其血浆浓度（两组均为10 pg/ml）无显著差异，患处皮肤中IL-17A的表达较低（分别为9119和19113，p = 0.036）。虽然我们注意到IL-23在PBMC中的表达增加（4419比808，p < 0.001），但结果未能证明morphea组IL-23血浆浓度比对照组升高（分别为5 pg/ml和6 pg/ml， p = 0.335）或其在皮肤中的表达增加（292比427，p = 0.383）。根据观察到的相关性，我们认为：(1)IL-17A不代表morphea中促进组织损伤的因素；(2)IL-23可能在morphea的发病机制中起作用。
Morphea is a disease included in the group of scleroderma type autoimmune diseases. Interleukin (IL)-17A may play a role at every stage of its pathogenesis. The study aimed at evaluation of IL-17A and IL-23 (as the main cytokine which is supposed to stimulate and maintain synthesis of IL-17) in pathogenesis of morphea. The studies were performed on 41 blood samples from patients with morphea. Skin was sampled from 29 patients. The evaluation included: (1) expression of IL-17A and IL-23 genes in peripheral blood mononuclear cells (PBMC) using real-time polymerase chain reaction (PCR), (2) plasma concentrations of IL-17A and IL-23 using ELISA, (3) expression of IL-17A and IL-23 genes in skin using real-time PCR. The results of gene expression are expressed as median number of copies per million copies of GAPDH. Higher expression of IL-17A has been demonstrated in PBMC of morphea vs. control group (2630 and 1906 respectively; p = 0.004), accompanied by absence of significant differences in its plasma concentration (10 pg/ml in both groups) and by lowered expression in affected skin (9119 and 19113 respectively; p = 0.036). The results failed to demonstrate elevated IL-23 plasma concentration in morphea vs. control group (5 pg/ml and 6 pg/ml respectively; p = 0.335) or its increased expression in the skin (292 vs. 427; p = 0.383), although we noted its increased expression in PBMC (4419 vs. 808; p < 0.001). Based on the observed correlations we suggest that: (1) IL-17A does not represent a factor which promotes tissue injury in morphea, (2) IL-23 may playa role in pathogenesis of morphea.
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作者：
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作者：
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DOI：
10.5114/aoms.2011.20610
发表时间：
2011-02
期刊：
Archives of medical science : AMS
影响因子：
--
作者：
Dziankowska-Bartkowiak B;Gerlicz-Kowalczuk Z;Waszczykowska E
通讯作者：
Waszczykowska E