Cooperation between translating ribosomes and RNA polymerase in transcription elongation.
Cooperation between translating ribosomes and RNA polymerase in transcription elongation.
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DOI：
10.1126/science.1184939
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发表时间：
2010-04-23
期刊：
影响因子：
--
通讯作者：
Nudler E
中科院分区：
文献类型：
--
作者：
Proshkin S;Rahmouni AR;Mironov A;Nudler E
在蛋白质编码基因的转录过程中，细菌RNA聚合酶（RNAP）紧随其后的是一个核糖体，该核糖体参与新合成的转录本的翻译。在这里，我们表明，作为一个结果，翻译-转录耦合的整体延伸率是严格控制的翻译。核糖体的加速和减速导致RNAP速度的相应变化。因此，我们发现基因中稀有密码子的数量与转录速率呈负相关，而稀有密码子会延迟核糖体的进展。我们进一步表明，核糖体对RNAP的刺激作用是通过防止RNAP采用非生产状态来实现的。移动的核糖体抑制RNAP的自发回溯，从而提高其速度，并促进体内路障的通读。这种合作机制确保两种基因表达机制彼此精确匹配，使得转录产量总是根据不同基因和各种生长条件下的翻译需求进行调整。
During transcription of protein-coding genes, bacterial RNA polymerase (RNAP) is closely followed by a ribosome that is engaged in translation of the newly synthesized transcript. Here we show that as a result of translation-transcription coupling the overall elongation rate of transcription is tightly controlled by translation. Acceleration and deceleration of a ribosome results in corresponding changes in the speed of RNAP. Consistently, we found an inverse correlation between the number of rare codons in a gene, which delay ribosome progression, and the rate of transcription. We further show that the stimulating effect of a ribosome on RNAP is achieved by preventing RNAP from adopting non-productive states. The moving ribosome inhibits spontaneous backtracking of RNAP, thereby enhancing its pace and also facilitating read-through of roadblocks in vivo. Such a cooperative mechanism ensures the two gene expression machineries match precisely each other rates, so that the transcriptional yield is always adjusted to translational needs at different genes and under various growth conditions.
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影响因子：
16.6
作者：
Nudler E
通讯作者：
Nudler E
影响因子：
16
作者：
Saeki, Hideaki;Svejstrup, Jesper Q.
通讯作者：
Svejstrup, Jesper Q.
影响因子：
16
作者：
Gusarov, I;Nudler, E
通讯作者：
Nudler, E
影响因子：
4.8
作者：
Komissarova, N;Kashlev, M
通讯作者：
Kashlev, M
DOI：
10.1007/bf00328706
发表时间：
1984-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
BOHMAN, K;RUUSALA, T;KURLAND, CG
通讯作者：
KURLAND, CG