Pm62, an adult-plant powdery mildew resistance gene introgressed from Dasypyrum villosum chromosome arm 2VL into wheat
Pm62, an adult-plant powdery mildew resistance gene introgressed from Dasypyrum villosum chromosome arm 2VL into wheat
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Pm62，从Dasypyrum villosum染色体臂2VL渗入小麦的成株白粉病抗性基因
DOI：
10.1007/s00122-018-3176-5
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发表时间：
2018-08
影响因子：
5.4
通讯作者：
Feng Yigao
中科院分区：
文献类型：
--
作者：
Zhang Ruiqi;Fan Yali;Kong Lingna;Wang Zuojun;Wu Jizhong;Xing Liping;Cao Aizhong;Feng Yigao
Pm 62是一个新的成株抗白粉病基因，它是从D.小麦白粉病是由小麦白粉病菌Blumeria graminis f.小麦赤霉病是小麦的一种主要病害，在世界上许多小麦生产地区造成产量和品质的严重损失。利用野生种的抗性基因渗入普通小麦，可有效控制该病的发生。硬粒小麦-簇毛麦2V#5染色体二体附加系N59 B-1（2n=30）在成株期提高了对白粉病的抗性，这归因于2V#5染色体。为了将该抗性转移到面包小麦中，通过组合基因组原位杂交和荧光原位杂交、2 V特异性标记分析以及对白粉病的反应来筛选来自N59 B-1与中国春杂交的总共298个BC 1F 1植株，以确认显性成株抗性基因，命名为Pm 62，位于染色体2VL#5上。随后，通过BC 1F 2代的分子和细胞遗传学分析鉴定了具有正常植物活力和完全育性的2VL#5（2D）二体置换系（NAU 1825）和纯合T2 BS. 2VL#5罗伯逊易位系（NAU 1823）。还在F-2分离群体中评估了T2 BS. 2VL#5重组染色体对农艺性状的影响。结果表明，易位染色体对株高、千粒重和花期无明显影响，但对穗长和穗粒数影响不大。易位系NAU 1823已作为新的抗白粉病种质资源在小麦抗白粉病育种中得到利用，T2 BS. 2VL#5重组染色体对小麦产量性状和面粉品质性状的影响有待进一步研究。
Key message Pm62, a novel adult-plant resistance (APR) gene against powdery mildew, was transferred from D. villosum into common wheat in the form of Robertsonian translocation T2BS.2VL#5.AbstractPowdery mildew, which is caused by the fungus Blumeria graminis f. sp. tritici, is a major disease of wheat resulting in substantial yield and quality losses in many wheat production regions of the world. Introgression of resistance from wild species into common wheat has application for controlling this disease. A Triticum durum-Dasypyrum villosum chromosome 2V#5 disomic addition line, N59B-1 (2n=30), improved resistance to powdery mildew at the adult-plant stage, which was attributable to chromosome 2V#5. To transfer this resistance into bread wheat, a total of 298 BC1F1 plants derived from the crossing between N59B-1 and Chinese Spring were screened by combined genomic in situ hybridization and fluorescent in situ hybridization, 2V-specific marker analysis, and reaction to powdery mildew to confirm that a dominant adult-plant resistance gene, designated as Pm62, was located on chromosome 2VL#5. Subsequently, the 2VL#5 (2D) disomic substitution line (NAU1825) and the homozygous T2BS.2VL#5 Robertsonian translocation line (NAU1823), with normal plant vigor and full fertility, were identified by molecular and cytogenetic analyses of the BC1F2 generation. The effects of the T2BS.2VL#5 recombinant chromosome on agronomic traits were also evaluated in the F-2 segregation population. The results suggest that the translocated chromosome may have no distinct effect on plant height, 1000-kernel weight or flowering period, but a slight effect on spike length and seeds per spike. The translocation line NAU1823 has being utilized as a novel germplasm in breeding for powdery mildew resistance, and the effects of the T2BS.2VL#5 recombinant chromosome on yield-related andflour quality characters will be further assessed.
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影响因子：
5.6
作者：
Bettgenhaeuser J;Gilbert B;Ayliffe M;Moscou MJ
通讯作者：
Moscou MJ
DOI：
--
发表时间：
2000
期刊：
Wheat Information Service
影响因子：
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DOI：
10.1071/9780643101463
发表时间：
1995
期刊：
--
影响因子：
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作者：
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通讯作者：
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影响因子：
3.7
作者：
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通讯作者：
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影响因子：
1.9
作者：
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通讯作者：
Gradzielewska, Agnieszka