Virulence Structure of Blumeria graminis f. sp. tritici and Its Genetic Diversity by ISSR and SRAP Profiling Analyses.
Virulence Structure of Blumeria graminis f. sp. tritici and Its Genetic Diversity by ISSR and SRAP Profiling Analyses.
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DOI：
10.1371/journal.pone.0130881
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yang C
中科院分区：
文献类型：
--
作者：
Liu N;Liu ZL;Gong G;Zhang M;Wang X;Zhou Y;Qi X;Chen H;Yang J;Luo P;Yang C
小麦白粉菌(Blumeria graminis f.sp.)小麦白粉病是一种专性生物营养病原菌，很容易在遗传上适应其寄主植物。了解小麦白粉病病原菌的毒力结构和遗传变异对小麦白粉病的防治和抗病育种具有重要意义。本研究对四川省中国地区的17个病原种群进行了调查，根据致病力分析将10 9个分离物分为强毒力(HV，92株)和低毒力(LV，17株)两大类群。宜宾(南部地区)、西昌(西部地区)和眉山(中部地区)种群的毒力频率低于其他地区的种群。在这项研究中，许多先前已知的抗性基因都没有赋予抗性。抗病基因Pm21对四川所有种群均表现免疫应答，Pm13、Pm5b、Pm2+6和PmXBD保持抗性。AMOVA显示，种群内的变异水平明显较高，而区域内种群间的变异水平较低。在这四个地区的人群中检测到了高水平的基因流动。利用ISSR和SRAP等位基因进行聚类分析，区分出每个地区的亲缘关系较近的群体。ISSR和SRAP等位基因图谱分析均显示四川病原种群具有较高的遗传多样性。尽管ISSR和SRAP图谱分析显示了相似的分辨率，但SRAP等位基因似乎提供了更多的信息。我们没有发现这些等位基因与病原体的毒力或致病性之间有任何显著的关联。我们的结果表明，ISSR和SRAP等位基因比远亲群体更能有效地表征小的或密切相关的群体。
Blumeria graminis f. sp. tritici, which causes wheat powdery mildew, is an obligate biotrophic pathogen that can easily genetically adapt to its host plant. Understanding the virulence structure of and genetic variations in this pathogen is essential for disease control and for breeding resistance to wheat powdery mildew. This study investigated 17 pathogenic populations in Sichuan, China and classified 109 isolates into two distinct groups based on pathogenicity analysis: high virulence (HV, 92 isolates) and low virulence (LV, 17 isolates). Populations from Yibin (Southern region), Xichang (Western region), and Meishan (Middle region) showed lower virulence frequencies than populations from other regions. Many of the previously known resistance genes did not confer resistance in this study. The resistance gene Pm21 displayed an immune response to pathogenic challenge with all populations in Sichuan, and Pm13, Pm5b, Pm2+6, and PmXBD maintained resistance. AMOVA revealed significantly higher levels of variation within populations and lower levels of variation among populations within regions. High levels of gene flow were detected among populations in the four regions. Closely related populations within each region were distinguished by cluster analyses using ISSR and SRAP alleles. Both ISSR and SRAP allele profiling analyses revealed high levels of genetic diversity among pathogenic populations in Sichuan. Although ISSR and SRAP profiling analysis showed similar resolutions, the SRAP alleles appeared to be more informative. We did not detect any significant association between these alleles and the virulence or pathogenicity of the pathogen. Our results suggest that ISSR and SRAP alleles are more efficient for the characterization of small or closely related populations versus distantly related populations.
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通讯作者：
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