Genome-wide identification, phylogeny and expression profile of vesicle fusion components in Verticillium dahliae.
Genome-wide identification, phylogeny and expression profile of vesicle fusion components in Verticillium dahliae.
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大丽黄萎病囊泡融合成分的全基因组鉴定、系统发育和表达谱
DOI：
10.1371/journal.pone.0068681
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Yang X;Ben S;Sun Y;Fan X;Tian C;Wang Y
囊泡运输在真核细胞的蛋白质定位和运动、信号转导以及多种发育过程中起着至关重要的作用。囊泡融合是囊泡转运的最后一步，主要依赖于可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体（SNARE）、SM（Sec 1/Munc 18）家族蛋白、Rab GTP酶和囊外亚基等调节因子。大丽轮枝菌是一种广泛存在的土壤真菌，会导致多种植物发生破坏性维管疾病。迄今为止，尚未在大丽轮枝菌中鉴定和表征参与囊泡融合过程的基因。最近发表的大丽轮枝菌基因组序列草图使我们能够对编码囊泡融合组分的基因进行全基因组鉴定、克隆和表达谱分析。利用比较基因组学和系统发育学方法，我们在大丽轮枝菌基因组中鉴定了44个编码囊泡融合组分的基因。根据编码蛋白的结构特征，将44个大丽轮枝菌基因分为22个SNARE（6个Qa-、4个Qb-、6个Qc-、1个Qbc-和5个R-型）、4个SM家族蛋白、10个Rab GTP酶和8个外囊蛋白。基于同源性和模体构成分析，丝状真菌中囊泡融合组分的直系同源物通常聚在一起，并具有良好支持的bootstrap值的相同亚类。对这些基因表达谱的分析表明，它们中的许多基因在大丽轮枝菌营养生长和微菌核形成过程中显著差异表达。分析表明，泡囊融合的许多成分在丝状真菌中保存良好，表明泡囊融合在烟树枯萎病真菌大丽轮枝菌微菌核形成中起着关键作用。对泡囊融合相关基因的全基因组鉴定和表达分析，将有助于对泡囊融合基因家族的研究，并为阐明泡囊融合基因在大丽轮枝菌生长发育和致病中的作用提供新的思路。
Vesicular trafficking plays a crucial role in protein localization and movement, signal transduction, and multiple developmental processes in eukaryotic cells. Vesicle fusion is the final and key step in vesicle-mediated trafficking and mainly relies on SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors), the regulators including SM (Sec1/Munc18) family proteins, Rab GTPases and exocyst subunits. Verticillium dahliae is a widespread soil fungus that causes disruptive vascular diseases on a wide range of plants. To date, no genes involved in vesicular fusion process have been identified and characterized in V. dahliae. The recent publication of the draft genome sequence of V. dahliae allowed us to conduct a genome-wide identification, phylogeny and expression profile of genes encoding vesicular fusion components. Using compared genomics and phylogenetic methods, we identified 44 genes encoding vesicle fusion components in the V. dahliae genome. According to the structural features of their encoded proteins, the 44 V. dahliae genes were classified into 22 SNAREs (6 Qa-, 4 Qb-, 6 Qc-, 1 Qbc- and 5 R-types), 4 SM family proteins, 10 Rab GTPases and 8 exocyst proteins. Based on phylogeny and motif constitution analysis, orthologs of vesicle fusion component in filamentous fungi were generally clustered together into the same subclasses with well-supported bootstrap values. Analysis of the expression profiles of these genes indicated that many of them are significantly differentially expressed during vegetative growth and microsclerotia formation in V. dahliae. The analysis show that many components of vesicle fusion are well conserved in filamentous fungi and indicate that vesicle fusion plays a critical role in microsclerotia formation of smoke tree wilt fungus V. dahliae. The genome-wide identification and expression analysis of components involved in vesicle fusion should facilitate research in this gene family and give new insights toward elucidating their functions in growth, development and pathogenesis of V. dahliae.
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DOI：
10.1007/bf00408881
发表时间：
1970-01-01
期刊：
ARCHIV FUR MIKROBIOLOGIE
影响因子：
--
作者：
GRIFFITHS, DA
通讯作者：
GRIFFITHS, DA
影响因子：
3.4
作者：
Kienle N;Kloepper TH;Fasshauer D
通讯作者：
Fasshauer D
影响因子：
3.7
作者：
Dou X;Wang Q;Qi Z;Song W;Wang W;Guo M;Zhang H;Zhang Z;Wang P;Zheng X
通讯作者：
Zheng X
影响因子：
3
作者：
Klimes, Anna;Amyotte, Stefan G.;Dobinson, Katherine F.
通讯作者：
Dobinson, Katherine F.
影响因子：
4
作者：
Elias, Marek;Brighouse, Andrew;Dacks, Joel B.
通讯作者：
Dacks, Joel B.