Bioavailable Microbial Metabolites of Flavanols Demonstrate Highly Individualized Bioactivity on In Vitro β-Cell Functions Critical for Metabolic Health.
Bioavailable Microbial Metabolites of Flavanols Demonstrate Highly Individualized Bioactivity on In Vitro β-Cell Functions Critical for Metabolic Health.
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DOI：
10.3390/metabo13070801
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发表时间：
2023-06-28
期刊：
影响因子：
4.1
通讯作者：
中科院分区：
文献类型：
--
作者：
众所周知，膳食黄烷醇具有预防疾病的特性，但通常吸收不良。肠道微生物组的黄烷醇代谢物具有更高的生物利用度，并可能发挥保护活性。使用从补充黄烷醇的大鼠尿液中提取的代谢物混合物，并使用或不使用抗生素处理，我们研究了它们对INS-1 832/13 β细胞葡萄糖刺激的胰岛素分泌（GSIS）能力的影响。我们测量了非刺激性（低）和刺激性（高）葡萄糖水平下的胰岛素分泌、胰岛素分泌诱导倍数和总胰岛素含量。我们进行了治疗水平的比较，个体水平的剂量反应，以及代谢物组成的应答者与非应答者的预测分析。虽然前两项分析未阐明给药效应，但28只动物中有9只动物的代谢产物显示出显著的剂量反应，与给药无关。反应者与非反应者的区别显示，无论治疗如何，天然黄烷醇和戊内酯的水平接近预测增强GSIS的意义。虽然治疗水平的模式是不可辨别的，我们得出的结论是，高个体间的变异性表明，代谢物生物活性对GSIS的能力是黄烷醇补充或抗生素治疗相关性较低，可能与独特的微生物组或代谢组的每只动物。这些发现表明黄烷醇代谢物的活性是个性化的，并指出需要个性化的营养实践。
Dietary flavanols are known for disease preventative properties but are often poorly absorbed. Gut microbiome flavanol metabolites are more bioavailable and may exert protective activities. Using metabolite mixtures extracted from the urine of rats supplemented with flavanols and treated with or without antibiotics, we investigated their effects on INS-1 832/13 β-cell glucose stimulated insulin secretion (GSIS) capacity. We measured insulin secretion under non-stimulatory (low) and stimulatory (high) glucose levels, insulin secretion fold induction, and total insulin content. We conducted treatment-level comparisons, individual-level dose responses, and a responder vs. non-responder predictive analysis of metabolite composition. While the first two analyses did not elucidate treatment effects, metabolites from 9 of the 28 animals demonstrated significant dose responses, regardless of treatment. Differentiation of responders vs. non-responder revealed that levels of native flavanols and valerolactones approached significance for predicting enhanced GSIS, regardless of treatment. Although treatment-level patterns were not discernable, we conclude that the high inter-individual variability shows that metabolite bioactivity on GSIS capacity is less related to flavanol supplementation or antibiotic treatment and may be more associated with the unique microbiome or metabolome of each animal. These findings suggest flavanol metabolite activities are individualized and point to the need for personalized nutrition practices.
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影响因子：
5.6
作者：
García-Conesa MT;Chambers K;Combet E;Pinto P;Garcia-Aloy M;Andrés-Lacueva C;de Pascual-Teresa S;Mena P;Konic Ristic A;Hollands WJ;Kroon PA;Rodríguez-Mateos A;Istas G;Kontogiorgis CA;Rai DK;Gibney ER;Morand C;Espín JC;González-Sarrías A
通讯作者：
González-Sarrías A
影响因子：
4.3
作者：
Draney C;Hobson AE;Grover SG;Jack BO;Tessem JS
通讯作者：
Tessem JS
DOI：
10.1016/j.jnutbio.2018.09.004
发表时间：
2018-12
期刊：
The Journal of nutritional biochemistry
影响因子：
--
作者：
Bitner BF;Ray JD;Kener KB;Herring JA;Tueller JA;Johnson DK;Tellez Freitas CM;Fausnacht DW;Allen ME;Thomson AH;Weber KS;McMillan RP;Hulver MW;Brown DA;Tessem JS;Neilson AP
通讯作者：
Neilson AP
影响因子：
5.2
作者：
Gonzalez-Sarrias, Antonio;Garcia-Villalba, Rocio;Carlos Espin, Juan
通讯作者：
Carlos Espin, Juan
影响因子：
5.2
作者：
Fernandez-Millan, Elisa;Cordero-Herrera, Isabel;Angeles Martin, Maria
通讯作者：
Angeles Martin, Maria