Induction of apoptosis in MCF-7 cells by the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus Malaysian strain AF2240.
Induction of apoptosis in MCF-7 cells by the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus Malaysian strain AF2240.
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DOI：
10.3892/or.2013.2573
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发表时间：
2013-09
期刊：
影响因子：
4.2
通讯作者：
Ideris A
中科院分区：
文献类型：
--
作者：
Ghrici M;El Zowalaty M;Omar AR;Ideris A
纽卡斯尔病病毒（NDV）可能通过诱导细胞凋亡发挥其自然发生的溶瘤作用。我们假设病毒通过血凝素-神经氨酸酶（HN）糖蛋白与细胞的结合可能不仅足以诱导细胞凋亡，而且诱导比亲本NDV AF 2240病毒更高的细胞凋亡水平。分析和定量NDV AF 2240对MCF-7人乳腺癌细胞中细胞凋亡的诱导。此外，还扩增了新城疫病毒（NDV）AF 2240株HN基因的全序列，并将其克隆到pDisplay真核表达载体中。HN基因的表达首先在转染的MCF-7细胞的细胞表面膜上检测到。分析并定量HN诱导转染的MCF-7细胞中的细胞凋亡。HN基因单独表达能够诱导MCF-7细胞凋亡，但与亲本NDV AF 2240株相比，HN基因是较弱的凋亡诱导剂。总之，NDV AF 2240株是比其重组HN基因更合适的抗肿瘤候选药物，除非后者通过额外的修饰进一步改进。
Newcastle disease virus (NDV) exerts its naturally occurring oncolysis possibly through the induction of apoptosis. We hypothesized that the binding of the virus to the cell via the hemagglutinin-neuraminidase (HN) glycoprotein may be sufficient to not only induce apoptosis but to induce a higher apoptosis level than the parental NDV AF2240 virus. NDV AF2240 induction of apoptosis in MCF-7 human breast cancer cells was analyzed and quantified. In addition, the complete HN gene of NDV strain AF2240 was amplified, sequenced and cloned into the pDisplay eukaryotic expression vector. HN gene expression was first detected at the cell surface membrane of the transfected MCF-7 cells. HN induction of apoptosis in transfected MCF-7 cells was analyzed and quantified. The expression of the HN gene alone was able to induce apoptosis in MCF-7 cells but it was a less potent apoptosis inducer compared to the parental NDV AF2240 strain. In conclusion, the NDV AF2240 strain is a more suitable antitumor candidate agent than its recombinant HN gene unless the latter is further improved by additional modifications.
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