Salmonella Enteritidis Subunit Vaccine Candidate Based on SseB Protein Co-Delivered with Simvastatin as Adjuvant.
Salmonella Enteritidis Subunit Vaccine Candidate Based on SseB Protein Co-Delivered with Simvastatin as Adjuvant.
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基于 SseB 蛋白与辛伐他汀作为佐剂共递送的肠炎沙门氏菌亚单位候选疫苗
DOI：
10.3390/pathogens11040443
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发表时间：
2022-04-07
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
肠道沙门氏菌（S.肠道菌群是一种重要的人畜共患病原体，可导致人类腹泻和全身感染以及动物死亡。这是一个全球性的重大公共卫生问题。迫切需要安全有效的疫苗来控制和预防沙门氏菌感染。亚单位疫苗是安全的，并提供针对沙门氏菌属的靶向保护。在这里，我们开发和评估了一个S。肠炎亚单位疫苗候选物，含辛伐他汀的rHis-SseB佐剂。我们从S.重组蛋白经大肠杆菌表达系统表达。Western blotting证实了重组蛋白rHis-SseB和rGST-SseB与沙门氏菌C50041抗血清的免疫反应性。在肌肉内接种的小鼠模型中，用rHis-SseB和辛伐他汀的共免疫显著增强血清中SseB特异性抗体滴度（体液免疫应答）和脾淋巴细胞增殖（细胞免疫应答）。用rHis-SseB和辛伐他汀联合免疫对随后的S.肠杆菌C50041菌株，并减少肝脏和脾脏中的细菌定植。这些发现为S.肠炎亚单位疫苗。
Salmonella enterica serovar Enteritidis (S. Enteritidis) is an important zoonotic pathogen that can lead to diarrhea and systemic infections in humans and mortality in animals. This is a major public health issue worldwide. Safe and effective vaccines are urgently needed to control and prevent Salmonella infection. Subunit vaccines are safe and provide targeted protection against Salmonella spp. Here, we developed and evaluated an S. Enteritidis subunit vaccine candidate, the rHis-SseB adjuvant with simvastatin. We amplified the SseB gene from S. Enteritidis C50041 genomic DNA and expressed the recombinant proteins rHis-SseB and rGST-SseB using the Escherichia coli system. Western blotting confirmed the immunoreactivity of recombinant proteins rHis-SseB and rGST-SseB with antisera against Salmonella Enteritidis C50041. In a mouse model of intramuscular vaccination, co-immunization with rHis-SseB and simvastatin significantly enhanced both the SseB-specific antibody titer in serum (humoral immune response) and splenic lymphocyte proliferation (cellular immune response). Co-immunization with rHis-SseB and simvastatin provided 60% protection against subsequent challenge with the S. Enteritidis C50041 strain and decreased bacterial colonization in the liver and spleen. These findings provide a basis for the development of an S. Enteritidis subunit vaccine.
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影响因子：
15.8
作者：
Havelaar AH;Kirk MD;Torgerson PR;Gibb HJ;Hald T;Lake RJ;Praet N;Bellinger DC;de Silva NR;Gargouri N;Speybroeck N;Cawthorne A;Mathers C;Stein C;Angulo FJ;Devleesschauwer B;World Health Organization Foodborne Disease Burden Epidemiology Reference Group
通讯作者：
World Health Organization Foodborne Disease Burden Epidemiology Reference Group
影响因子：
6
作者：
Lee JH
通讯作者：
Lee JH
影响因子：
6.4
作者：
Reynolds CJ;Jones C;Blohmke CJ;Darton TC;Goudet A;Sergeant R;Maillere B;Pollard AJ;Altmann DM;Boyton RJ
通讯作者：
Boyton RJ
影响因子：
2.6
作者：
Lin Z;Tang P;Jiao Y;Kang X;Li Q;Xu X;Sun J;Pan Z;Jiao X
通讯作者：
Jiao X
影响因子：
2.8
作者：
Casarin Penha Filho, Rafael Antonio;de Paiva, Jacqueline Boldrin;Sesti, Luiz
通讯作者：
Sesti, Luiz