The Composition of Plant Mitochondrial Supercomplexes Changes with Oxygen Availability*
The Composition of Plant Mitochondrial Supercomplexes Changes with Oxygen Availability*
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植物线粒体超级复合物的组成随氧气利用率的变化而变化*
DOI：
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发表时间：
2011
影响因子：
4.8
通讯作者：
Joost T. van Dongen
中科院分区：
文献类型：
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作者：
S. J. Ramírez;Mandy Keuthe;M. Rocha;V. Fedyaev;Katharina Kramp;K. J. Gupta;A. Rasmusson;W. Schulze;Joost T. van Dongen
背景：呼吸超复合物是已知存在的，但其功能仍有待揭示。结果如下：植物超复合物的影响，缺氧和伴随的pH值下降。结论：呼吸超复合物是动态的结构，受到细胞内环境的影响。意义：超复合物可能具有引导电子通过替代呼吸途径的调节功能。呼吸超复合物是由线粒体电子传递链的各种酶复合物形成的大型蛋白质结构。使用天然凝胶电泳和活性染色，超复合物内的复合物活性的差异调节进行了研究。在长时间的缺氧，复合物I内的活动supercomplex减少，而单个复合物I-单体的活性增加。同时，在缺氧期间观察到不含复合物I的较小超复合物中复合物IV的活性增加。这些变化在复合物的活性内的supercomplex恢复过程中再次从缺氧处理。酸化的线粒体基质诱导类似的变化，在复杂的活动内的supercomplex。有人建议，小的超复合物III 2 +IV的活性增加可以解释由复合物I从大的超复合物的解离。这是讨论的一部分，调节参与的替代NADH脱氢酶，已知被激活的低pH值，和复合物I，这是抑制低pH值的机制。它的结论是，supercomplex内的复合物的活性可以调节取决于线粒体基质的氧状态和pH值。
Background: Respiratory supercomplexes are known to exist, but their function remains to be revealed. Results: Plant supercomplexes are affected by hypoxia and a concomitant drop in pH. Conclusion: Respiratory supercomplexes are dynamic structures that are affected by the intracellular environment. Significance: Supercomplexes could have a regulatory function in guiding electrons through alternative respiratory pathways. Respiratory supercomplexes are large protein structures formed by various enzyme complexes of the mitochondrial electron transport chain. Using native gel electrophoresis and activity staining, differential regulation of complex activity within the supercomplexes was investigated. During prolonged hypoxia, complex I activity within supercomplexes diminished, whereas the activity of the individual complex I-monomer increased. Concomitantly, an increased activity was observed during hypoxia for complex IV in the smaller supercomplexes that do not contain complex I. These changes in complex activity within supercomplexes reverted again during recovery from the hypoxic treatment. Acidification of the mitochondrial matrix induced similar changes in complex activity within the supercomplexes. It is suggested that the increased activity of the small supercomplex III2+IV can be explained by the dissociation of complex I from the large supercomplexes. This is discussed to be part of a mechanism regulating the involvement of the alternative NADH dehydrogenases, known to be activated by low pH, and complex I, which is inhibited by low pH. It is concluded that the activity of complexes within supercomplexes can be regulated depending on the oxygen status and the pH of the mitochondrial matrix.
影响因子：
3.9
作者：
Jensen,BD;Gunter,KK;Gunter,TE
通讯作者：
Gunter,TE
影响因子：
4.2
作者：
van Dongen JT;Fröhlich A;Ramírez-Aguilar SJ;Schauer N;Fernie AR;Erban A;Kopka J;Clark J;Langer A;Geigenberger P
通讯作者：
Geigenberger P
DOI：
10.1073/pnas.95.12.6803
发表时间：
1998-06-09
影响因子：
11.1
作者：
Llopis, J;McCaffery, JM;Tsien, RY
通讯作者：
Tsien, RY