Transcript and metabolite profiling of the adaptive response to mild decreases in oxygen concentration in the roots of arabidopsis plants.
Transcript and metabolite profiling of the adaptive response to mild decreases in oxygen concentration in the roots of arabidopsis plants.
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DOI：
10.1093/aob/mcn126
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发表时间：
2009-01
期刊：
影响因子：
4.2
通讯作者：
Geigenberger P
中科院分区：
文献类型：
--
作者：
van Dongen JT;Fröhlich A;Ramírez-Aguilar SJ;Schauer N;Fernie AR;Erban A;Kopka J;Clark J;Langer A;Geigenberger P
氧在植物组织中的浓度很低，这要么是因为环境因素降低了外部氧浓度，要么是因为氧在植物组织中的移动不能跟上氧气消耗的速度。最近的研究证明，植物可以根据氧气浓度的相对较小的变化来减少它们的氧气消耗，以避免内部缺氧。这种反应背后的分子机制尚未确定。这项研究的目的是利用转录本和代谢物图谱来研究拟南芥根对氧浓度轻微下降的基因组反应。用21、8、4和1%(v/v)的外源氧气在垂直平板上培养拟南芥幼苗0·5、2和48h，用Affymetrix全基因组DNA芯片分析根转录水平的变化，并用常规的GC-MS代谢物图谱分析代谢物水平的变化。同时监测了根伸长率，以调查生长的适应性变化。结果表明，根的生长受到抑制，转录物和代谢产物谱显着改变，以响应适度的氧气浓度下降。低氧导致基因的优先上调，这可能是触发植物适应性反应的重要因素。一组小但高度特异的基因很早就被诱导出来，以响应氧气浓度的适度下降。下调的基因主要编码与能量消耗过程有关的蛋白质。与此相一致的是，根伸长生长显著减少，最终将节省ATP，降低耗氧量。伴随而来的是在低氧条件下短期和长期孵育的代谢物水平的不同调节。结果表明，当氧气浓度在很小的范围内降低时，根伸长发生了适应性的变化，涉及到基因表达和代谢的大规模重新编程。
Oxygen can fall to low concentrations within plant tissues, either because of environmental factors that decrease the external oxygen concentration or because the movement of oxygen through the plant tissues cannot keep pace with the rate of oxygen consumption. Recent studies document that plants can decrease their oxygen consumption in response to relatively small changes in oxygen concentrations to avoid internal anoxia. The molecular mechanisms underlying this response have not been identified yet. The aim of this study was to use transcript and metabolite profiling to investigate the genomic response of arabidopsis roots to a mild decrease in oxygen concentrations. Arabidopsis seedlings were grown on vertical agar plates at 21, 8, 4 and 1 % (v/v) external oxygen for 0·5, 2 and 48 h. Roots were analysed for changes in transcript levels using Affymetrix whole genome DNA microarrays, and for changes in metabolite levels using routine GC-MS based metabolite profiling. Root extension rates were monitored in parallel to investigate adaptive changes in growth. The results show that root growth was inhibited and transcript and metabolite profiles were significantly altered in response to a moderate decrease in oxygen concentrations. Low oxygen leads to a preferential up-regulation of genes that might be important to trigger adaptive responses in the plant. A small but highly specific set of genes is induced very early in response to a moderate decrease in oxygen concentrations. Genes that were down-regulated mainly encoded proteins involved in energy-consuming processes. In line with this, root extension growth was significantly decreased which will ultimately save ATP and decrease oxygen consumption. This was accompanied by a differential regulation of metabolite levels at short- and long-term incubation at low oxygen. The results show that there are adaptive changes in root extension involving large-scale reprogramming of gene expression and metabolism when oxygen concentration is decreased in a very narrow range.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
11.6
作者：
Klok, EJ;Wilson, IW;Dennis, ES
通讯作者：
Dennis, ES
影响因子：
7.2
作者：
Gibon, Y;Vigeolas, H;Stitt, M
通讯作者：
Stitt, M
影响因子：
4.2
作者：
Gonzali, S;Loreti, E;Perata, P
通讯作者：
Perata, P
影响因子：
6.9
作者：
Jackson, MB
通讯作者：
Jackson, MB