A preferential pole for exocytosis in cultured chromaffin cells revealed by confocal microscopy
A preferential pole for exocytosis in cultured chromaffin cells revealed by confocal microscopy
复制标题
共聚焦显微镜揭示培养嗜铬细胞胞吐作用的优先极
DOI：
10.1016/s0014-5793(99)01115-1
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发表时间：
1999
期刊：
影响因子：
3.5
通讯作者：
Antonio G. García
中科院分区：
文献类型：
--
作者：
I. Cuchillo;P. Michelena;A. Albillos;Antonio G. García
Histological studies suggest that adrenal medulla chromaffin cells in situ are polarised, but functional evidence is lacking. We present here the first demonstration for polarisation of exocytosis in isolated, spherical, bovine chromaffin cells. Cells were stimulated with 70 mM K+to cause a marked enhancement of catecholamine release, monitored amperometrically. FM1-43 and dopamine β-hydroxylase antibodies provided fluorescence confocal pictures that were 2–3-fold more intense in the bottom of the cells, as compared to equatorial or apex planes. This suggests that the solid phase to which the cell attaches serves as a ‘trophic’ signal for the polarisation of its secretory apparatus.
影响因子：
56.9
作者：
BETZ, WJ;BEWICK, GS
通讯作者：
BEWICK, GS
影响因子：
5.5
作者：
AUGUSTINE, GJ;NEHER, E
通讯作者：
NEHER, E