Tal1, Gata2a, and Gata3 Have Distinct Functions in the Development of V2b and Cerebrospinal Fluid-Contacting KA Spinal Neurons.
Tal1, Gata2a, and Gata3 Have Distinct Functions in the Development of V2b and Cerebrospinal Fluid-Contacting KA Spinal Neurons.
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DOI：
10.3389/fnins.2018.00170
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发表时间：
2018
影响因子：
4.3
通讯作者：
Lewis KE
中科院分区：
文献类型：
--
作者：
Andrzejczuk LA;Banerjee S;England SJ;Voufo C;Kamara K;Lewis KE
脊椎动物的运动回路包含不同类别的腹侧脊髓神经元，每个神经元具有特定的功能特性。虽然我们知道这些细胞类型中的每一种表达的一些基因，但我们还不知道这些神经元中的几种是如何被指定的。在这里，我们研究了Tal 1，Gata 2a和Gata 3转录因子在运动回路中具有重要作用的两个神经元群体的发育中的功能：V2 b神经元和脑脊液接触Kolmer-Agduhr（KA）神经元（也称为CSF-cNs）。我们的数据首次证明，在任何脊椎动物中，Tal 1和Gata 3分别是KA和V2 b神经元正确发育所需的。我们还发现了斑马鱼与小鼠V2 b细胞发育的遗传调控差异。此外，我们证明，Sox 1a和Sox 1b的表达KA和V2 b神经元在斑马鱼，这不同于小鼠，其中Sox 1的表达V2 c神经元。KA神经元可分为腹侧KA″神经元和背侧KA′神经元。与以前的吗啉实验一致，我们的突变数据表明，Tal 1和Gata 3是KA '细胞所需的，而不是KA“细胞，而Gata 2a是KA”细胞所需的，而不是KA'细胞，即使这两种细胞类型共表达所有这三种转录因子。在gata 2a突变体中，脊髓KA″区域的细胞失去了大多数KA″基因的表达，并且表达V3基因的细胞数量增加，这表明Gata 2a需要指定KA″并抑制正常发育为KA″神经元的细胞中的V3命运。另一方面，我们的数据表明Gata 3和Tal 1都是KA′神经元从祖细胞分化所必需的。在这些突变体的KA′区域，细胞不再表达KA′标记，并且有丝分裂活性细胞的数量增加。最后，我们的数据表明，所有这三个转录因子所需的V2 b神经元分化的后期阶段，Gata 2a和Tal 1在斑马鱼V2 b的发展比在小鼠中有不同的功能。
Vertebrate locomotor circuitry contains distinct classes of ventral spinal cord neurons which each have particular functional properties. While we know some of the genes expressed by each of these cell types, we do not yet know how several of these neurons are specified. Here, we investigate the functions of Tal1, Gata2a, and Gata3 transcription factors in the development of two of these populations of neurons with important roles in locomotor circuitry: V2b neurons and cerebrospinal fluid-contacting Kolmer-Agduhr (KA) neurons (also called CSF-cNs). Our data provide the first demonstration, in any vertebrate, that Tal1 and Gata3 are required for correct development of KA and V2b neurons, respectively. We also uncover differences in the genetic regulation of V2b cell development in zebrafish compared to mouse. In addition, we demonstrate that Sox1a and Sox1b are expressed by KA and V2b neurons in zebrafish, which differs from mouse, where Sox1 is expressed by V2c neurons. KA neurons can be divided into ventral KA″ neurons and more dorsal KA′ neurons. Consistent with previous morpholino experiments, our mutant data suggest that Tal1 and Gata3 are required in KA′ but not KA″ cells, whereas Gata2a is required in KA″ but not KA′ cells, even though both of these cell types co-express all three of these transcription factors. In gata2a mutants, cells in the KA″ region of the spinal cord lose expression of most KA″ genes and there is an increase in the number of cells expressing V3 genes, suggesting that Gata2a is required to specify KA″ and repress V3 fates in cells that normally develop into KA″ neurons. On the other hand, our data suggest that Gata3 and Tal1 are both required for KA′ neurons to differentiate from progenitor cells. In the KA′ region of these mutants, cells no longer express KA′ markers and there is an increase in the number of mitotically-active cells. Finally, our data demonstrate that all three of these transcription factors are required for later stages of V2b neuron differentiation and that Gata2a and Tal1 have different functions in V2b development in zebrafish than in mouse.
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影响因子：
4.6
作者：
Cheesman, SE;Layden, MJ;Eisen, JS
通讯作者：
Eisen, JS
影响因子：
4.6
作者：
Craven, SE;Lim, KC;Rosenthal, A
通讯作者：
Rosenthal, A
影响因子：
2.5
作者：
BERNHARDT, RR;PATEL, CK;KUWADA, JY
通讯作者：
KUWADA, JY
影响因子：
3.6
作者：
Hilinski WC;Bostrom JR;England SJ;Juárez-Morales JL;de Jager S;Armant O;Legradi J;Strähle U;Link BA;Lewis KE
通讯作者：
Lewis KE
DOI：
10.1242/dev.145318
发表时间：
2017-03-01
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Home P;Kumar RP;Ganguly A;Saha B;Milano-Foster J;Bhattacharya B;Ray S;Gunewardena S;Paul A;Camper SA;Fields PE;Paul S
通讯作者：
Paul S