Evaluation of zona pellucida function for sperm penetration during in vitro fertilization in pigs.
Evaluation of zona pellucida function for sperm penetration during in vitro fertilization in pigs.
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DOI：
10.1262/jrd.2013-021
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Kikuchi K
中科院分区：
文献类型：
--
作者：
Tanihara F;Nakai M;Kaneko H;Noguchi J;Otoi T;Kikuchi K
在猪卵母细胞中，透明带（ZP）在精子穿透或防止多精受精方面的功能还不清楚。在本研究中，我们研究了ZP在体外受精（IVF）过程中对精子穿透的影响。我们收集了具有第一极体的体外成熟卵母细胞（ZP+卵母细胞）。通过两种处理（链霉蛋白酶和机械移液）将其中一些从ZP（ZP−卵母细胞）中释放出来，并评估这些处理对精子穿透参数（精子穿透率和每个卵母细胞穿透精子的数量）的影响。两组间各项参数无明显差异。其次，我们比较了精子穿透参数的ZP+和ZP−卵母细胞使用冻融附睾精子从四个公猪。精子进入ZP+卵母细胞的速度比ZP−卵母细胞快。第三，我们评估了IVF后1 - 10 h（3 h配子共孵育）ZP+和ZP−卵母细胞的精子穿透率。在两组中，精子穿透卵母细胞的比例随着时间的推移而显著增加;然而，在ZP−卵母细胞中，每个卵母细胞穿透的精子数量没有增加。最后，我们使用ZP−卵母细胞进行IVF，分为对照组（3 h）和延长配子共孵育组（5 h）。5 h组精子穿透率高于对照组。这些结果表明，ZP和卵膜是不称职的因素，以防止多精受精在我们目前的猪IVF系统。然而，似乎ZP去除是减少猪体外多精穿透的可能性之一。
In porcine oocytes, the function of the zona pellucida (ZP) with regard to sperm penetration or prevention of polyspermy is not well understood. In the present study, we investigated the effects of the ZP on sperm penetration during in vitro fertilization (IVF). We collected in vitro-matured oocytes with a first polar body (ZP+ oocytes). Some of them were freed from the ZP (ZP− oocytes) by two treatments (pronase and mechanical pipetting), and the effects of these treatments on sperm penetration parameters (sperm penetration rate and numbers of penetrated sperm per oocyte) were evaluated. There was no evident difference in the parameters between the two groups. Secondly, we compared the sperm penetration parameters of ZP+ and ZP− oocytes using frozen-thawed epididymal spermatozoa from four boars. Sperm penetration into ZP+ oocytes was found to be accelerated relative to ZP− oocytes. Thirdly, we evaluated the sperm penetration of ZP+ and ZP− oocytes at 1−10 h after IVF (3 h gamete co-incubation). The proportions of oocytes penetrated by sperm increased significantly with time in both groups; however, the number of penetrated sperm per oocyte did not increase in ZP− oocytes. Finally, we performed IVF using ZP− oocytes divided into control (3 h) and prolonged gamete co-incubation (5 h) groups. Greater numbers of sperm penetrated in the 5 h group than in the control group. These results suggest that the ZP and oolemma are not competent factors for prevention of polyspermy in our present porcine IVF system. However, it appears that ZP removal is one of the possibilities for reducing polyspermic penetration in vitro in pigs.
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影响因子：
3.8
作者：
Funahashi, H;Ekwall, H;Rodriguez-Martinez, H
通讯作者：
Rodriguez-Martinez, H
DOI：
10.1530/jrf.0.1070079
发表时间：
1996-05-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
Kim, NH;Funahashi, H;Day, BN
通讯作者：
Day, BN
DOI：
10.1073/pnas.0804422105
发表时间：
2008-10-14
影响因子：
11.1
作者：
Coy, Pilar;Canovas, Sebastian;Aviles, Manuel
通讯作者：
Aviles, Manuel
影响因子：
3.8
作者：
Canovas, Sebastian;Romar, Raquel;Coy, Pilar
通讯作者：
Coy, Pilar
影响因子：
3.6
作者：
IMAI, H;NIWA, K;IRITANI, A
通讯作者：
IRITANI, A