Multiomics studies with co-transformation reveal microRNAs via miRNA-TF-mRNA network participating in wood formation in Hevea brasiliensis.
Multiomics studies with co-transformation reveal microRNAs via miRNA-TF-mRNA network participating in wood formation in Hevea brasiliensis.
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DOI：
10.3389/fpls.2023.1068796
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发表时间：
2023
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
microRNAs（miRNAs）是一类在植物木材形成过程中起重要作用的内源性小分子非编码RNA。然而，miRNAs与其靶转录物之间的联系在橡胶树（Hevea brasiliensis）木材形成中的意义尚不清楚。在这项研究中，我们通过人工弯曲橡胶树300天来诱导反应木的形成，并进行了小RNA测序和转录组深度测序（RNA-seq），以描述参与这一过程的miRNA及其靶标的互补。我们确定了5，11，和2个差异丰富的miRNA在正常木材（NW）相比，张力木材（TW），在NW相对于对向木材（OW），和TW和OW之间，分别。我们还在NW、TW和OW中鉴定了12种新型miRNA和39种潜在的miRNA-mRNA对，具有不同的积累模式。我们注意到许多miRNA靶向转录因子基因，这些基因在与苯丙烷类生物合成、苯丙氨酸代谢和丙酮酸代谢相关的KEGG途径中富集。因此，通过张力木材模型构建了参与木材形成的miRNA-TF-mRNA网络。我们通过RT-qPCR分析验证了miRNA及其靶基因的差异积累，并在本氏烟草中过量表达了miRNA及其潜在的靶基因。这些结果将为深入研究橡胶树的生长发育规律提供参考。
MicroRNAs (miRNAs) are small endogenous non-coding RNAs that play an important role in wood formation in plants. However, the significance of the link between miRNAs and their target transcripts in wood formation remains unclear in rubber tree (Hevea brasiliensis). In this study, we induced the formation of reaction wood by artificially bending rubber trees for 300 days and performed small RNA sequencing and transcriptome deep sequencing (RNA-seq) to describe the complement of miRNAs and their targets contributing to this process. We identified 5, 11, and 2 differentially abundant miRNAs in normal wood (NW) compared to tension wood (TW), in NW relative to opposite wood (OW), and between TW and OW, respectively. We also identified 12 novel miRNAs and 39 potential miRNA-mRNA pairs with different accumulation patterns in NW, TW, and OW. We noticed that many miRNAs targeted transcription factor genes, which were enriched in KEGG pathways associated with phenylpropanoid biosynthesis, phenylalanine metabolism, and pyruvate metabolism. Thus, miRNA-TF-mRNA network involved in wood formation via tension wood model were constructed. We validated the differential accumulation of miRNAs and their targets by RT-qPCR analysis and overexpressed miRNA in Nicotiana benthamiana with its potential target gene. These results will provide a reference for a deep exploration of growth and development in rubber tree.
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影响因子：
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作者：
Herrero, Joaquin;Fernandez-Perez, Francisco;Miguel Zapata, Jose
通讯作者：
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影响因子：
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作者：
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