Conservation and divergence of microRNAs and their functions in Euphorbiaceous plants.
Conservation and divergence of microRNAs and their functions in Euphorbiaceous plants.
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DOI：
10.1093/nar/gkp1035
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发表时间：
2010-01
影响因子：
14.9
通讯作者：
Peng M
中科院分区：
文献类型：
--
作者：
Zeng C;Wang W;Zheng Y;Chen X;Bo W;Song S;Zhang W;Peng M
microRNA（miRNAs）是一类长度为21 nt的非编码RNA，可调控转录后基因的表达。miRNAs是几乎所有重要生物过程的关键调节因子。为了深入了解大戟科植物中miRNA的功能，克服大戟科植物基因组缺乏的困难，采用计算预测和实验分析相结合的方法，对大戟科植物中miRNA进行了全基因组水平的系统研究。具体而言，我们预测了蓖麻（蓖麻）中23个家族中的85个保守的miRNA，并在正常幼苗发育过程中，在四种大戟科植物（蓖麻，木薯，橡胶树和麻风树）中的至少一种中实验验证和表征了85个miRNA中的58个（68.2%）。为了阐明它们在胁迫反应中的功能，我们在寒冷和干旱胁迫以及胁迫恢复过程中验证和分析了85个miRNAs中的48个（56.5%）。结果揭示了一些物种和条件特异性的miRNA表达模式。最后，我们预测了258个miRNA：目标伴侣，并通过改进的5′ RACE鉴定了10个miRNA靶中的6个的切割位点。本研究首次收集了大戟科植物的miRNAs及其靶基因。我们的研究结果揭示了大戟科植物在幼苗生长和非生物胁迫反应中许多miRNAs的广泛保守性和多样性功能。
MicroRNAs (miRNAs) are ∼21 nt non-coding RNAs which regulate post-transcriptional gene expression. miRNAs are key regulators of nearly all essential biological processes. Aiming at understanding miRNA’s functions in Euphorbiaceae, a large flowering plant family, we performed a genome-scale systematic study of miRNAs in Euphorbiaceae, by combining computational prediction and experimental analysis to overcome the difficulty of lack of genomes for most Euphorbiaceous species. Specifically, we predicted 85 conserved miRNAs in 23 families in the Castor bean (Ricinus communis), and experimentally verified and characterized 58 (68.2%) of the 85 miRNAs in at least one of four Euphorbiaceous species, the Castor bean, the Cassava (Manihot esculenta), the Rubber tree (Hevea brasiliensis) and the Jatropha (Jatropha curcas) during normal seedling development. To elucidate their function in stress response, we verified and profiled 48 (56.5%) of the 85 miRNAs under cold and drought stresses as well as during the processes of stress recovery. The results revealed some species- and condition-specific miRNA expression patterns. Finally, we predicted 258 miRNA:target partners, and identified the cleavage sites of six out of ten miRNA targets by a modified 5′ RACE. This study produced the first collection of miRNAs and their targets in Euphorbiaceae. Our results revealed wide conservation of many miRNAs and diverse functions in Euphorbiaceous plants during seedling growth and in response to abiotic stresses.
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影响因子：
56.9
作者：
Farh, KKH;Grimson, A;Bartel, DP
通讯作者：
Bartel, DP
影响因子：
5.1
作者：
Dugas, Diana V.;Bartel, Bonnie
通讯作者：
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影响因子：
16
作者：
Jones-Rhoades, MW;Bartel, DP
通讯作者：
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影响因子：
48
作者：
Hammell, Molly;Long, Dang;Zhang, Liang;Lee, Andrew;Carmack, C. Steven;Han, Min;Ding, Ye;Ambros, Victor
通讯作者：
Ambros, Victor
影响因子：
3.5
作者：
Guo, An-Yuan;Zhu, Qi-Hui;Luo, Jingchu
通讯作者：
Luo, Jingchu