The RNA binding protein RBPMS is a selective marker of ganglion cells in the mammalian retina.
The RNA binding protein RBPMS is a selective marker of ganglion cells in the mammalian retina.
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RNA 结合蛋白 RBPMS 是哺乳动物视网膜神经节细胞的选择性标记。
DOI：
10.1002/cne.23521
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发表时间：
2014-04-15
影响因子：
2.5
通讯作者：
Brecha, Nicholas C.
中科院分区：
文献类型：
--
作者：
Rodriguez, Allen R.;Mueller, Luis Perez de Sevilla;Brecha, Nicholas C.
有几个神经化学标记物，可靠地识别视网膜神经节细胞（RGC），这是一个异质群体的细胞，整合和传输的视觉信号从视网膜到中央视觉核。我们已经开发并表征了一组新的亲和纯化的豚鼠和兔抗多剪接RNA结合蛋白（RBPMS）的抗体。在Western印迹上，这些抗体识别约24 kDa处的单一条带，对应于RBPMS，并且它们强烈标记小鼠、大鼠、豚鼠、兔和猴视网膜中的RGC和移位的RGC（dRGC）胞体。RBPMS免疫反应性细胞和RGCs通过其他技术鉴定具有相似的染色体直径和面积范围。小鼠和大鼠视网膜中RBPMS细胞的密度与早期RGC的半定量估计相当。RBPMS主要在神经节细胞层（GCL）中的中型和大型DAPI-、DRAQ 5-、NeuroTrace-和NeuN-染色的细胞中表达，并且RBPMS不在内核层（INL）和GCL中的突触融合蛋白（HPC-1）免疫反应性细胞中表达，这与它们作为RGCs的身份一致，而不是移位的无长突细胞。在小鼠和大鼠视网膜中，大多数RBPMS细胞在视神经压碎或横断后三周丢失，并且所有Brn 3a、SMI-32和黑视素免疫反应性RGCs也表达RBPMS免疫反应性。RBPMS免疫反应性定位于B6.Cg-Tg（Thy 1-CFP）23 Jrs/J小鼠系中的CFP荧光RGCs。这些发现表明，针对RBPMS的抗体是专门鉴定多种哺乳动物物种中的RGC和dRGC的稳健试剂，并且它们将特别用于RGC的定量。
There are few neurochemical markers that reliably identify retinal ganglion cells (RGCs), which are a heterogeneous population of cells that integrate and transmit the visual signal from the retina to the central visual nuclei. We have developed and characterized a new set of affinity purified guinea pig and rabbit antibodies against RNA-binding protein with multiple splicing (RBPMS). On Western blots these antibodies recognize a single band at ~24 kDa, corresponding to RBPMS, and they strongly label RGC and displaced RGC (dRGC) somata in mouse, rat, guinea pig, rabbit and monkey retina. RBPMS immunoreactive cells and RGCs identified by other techniques have a similar range of somal diameters and areas. The density of RBPMS cells in mouse and rat retina is comparable to earlier semi-quantitative estimates of RGCs. RBPMS is mainly expressed in medium and large DAPI-, DRAQ5-, NeuroTrace- and NeuN-stained cells in the ganglion cell layer (GCL), and RBPMS is not expressed in syntaxin (HPC-1) immunoreactive cells in the inner nuclear layer (INL) and GCL, consistent with their identity as RGCs, and not displaced amacrine cells. In mouse and rat retina, most RBPMS cells are lost following optic nerve crush or transection at three weeks, and all Brn3a, SMI-32 and melanopsin immunoreactive RGCs also express RBPMS immunoreactivity. RBPMS immunoreactivity is localized to CFP-fluorescent RGCs in the B6.Cg-Tg(Thy1-CFP)23Jrs/J mouse line. These findings show that antibodies against RBPMS are robust reagents that exclusively identify RGCs and dRGCs in multiple mammalian species, and they will be especially useful for quantification of RGCs.
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影响因子：
64.8
作者：
Do, Michael Tri H.;Kang, Shin H.;Xue, Tian;Zhong, Haining;Liao, Hsi-Wen;Bergles, Dwight E.;Yau, King-Wai
通讯作者：
Yau, King-Wai
影响因子：
3.4
作者：
Dedek, Karin;Breuninger, Tobias;Weiler, Reto
通讯作者：
Weiler, Reto
影响因子：
2.5
作者：
DOI, M;UJI, Y;YAMAMURA, H
通讯作者：
YAMAMURA, H
影响因子：
64.8
作者：
BRECHA, N;JOHNSON, D;LIEBERMAN, AR
通讯作者：
LIEBERMAN, AR
影响因子：
2.5
作者：
BUHL, EH;DANN, JF
通讯作者：
DANN, JF