Agouti C57BL/6N embryonic stem cells for mouse genetic resources.
Agouti C57BL/6N embryonic stem cells for mouse genetic resources.
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DOI:
10.1038/nmeth.1342
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发表时间:
2009-07
期刊:
影响因子:
48
通讯作者:
Skarnes, William C.
中科院分区:
文献类型:
--
作者:
Pettitt, Stephen J.;Liang, Qi;Rairdan, Xin Y.;Moran, Jennifer L.;Prosser, Haydn M.;Beier, David R.;Lloyd, Kent C.;Bradley, Allan;Skarnes, William C.
We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant Agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.
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