Identification of distinct N-glycosylation patterns on extracellular vesicles from small-cell and non-small-cell lung cancer cells.
Identification of distinct N-glycosylation patterns on extracellular vesicles from small-cell and non-small-cell lung cancer cells.
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DOI：
10.1016/j.jbc.2022.101950
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发表时间：
2022-06
影响因子：
4.8
通讯作者：
Inoue, Hiromasa
中科院分区：
文献类型：
--
作者：
Kondo, Kiyotaka;Harada, Yoichiro;Nakano, Miyako;Suzuki, Takehiro;Fukushige, Tomoko;Hanzawa, Ken;Yagi, Hirokazu;Takagi, Koichi;Mizuno, Keiko;Miyamoto, Yasuhide;Taniguchi, Naoyuki;Kato, Koichi;Kanekura, Takuro;Dohmae, Naoshi;Machida, Kentaro;Maruyama, Ikuro;Inoue, Hiromasa
作为一种潜在的癌症检测和诊断的生物标志物，肿瘤分泌组蛋白的天冬酰胺连接糖基化（n -糖基化）越来越受到关注。小的细胞外囊泡（sev）构成了癌症分泌组的很大一部分，但它们的n -糖基化状态是否反映了已知的癌症特征尚不清楚。在这里，我们研究了小细胞肺癌（SCLC）和非小细胞肺癌（NSCLC）细胞释放的sev的n -糖基化。我们发现sclc - sev的n -聚糖的特征是存在与脑n -聚糖相同的结构单元，而nsclc - sev主要是典型的肺型n -聚糖，具有nsclc相关的核心聚焦。此外，凝集素辅助的sclc - sev和nsclc - sev的n-糖蛋白组学结果显示，整合素αV在两种癌细胞类型的sev中均有表达，而上皮特异性整合素α6β4异源二聚体在nsclc - sev中有选择性表达。重要的是，来自nsclc - sev的免疫纯化整合素α6的n糖组学鉴定出该整合素亚基上的nsclc型n聚糖。因此，我们得出结论，肺癌sev中的蛋白n -糖基化可能潜在地反映了肺癌的组织学。
Asparagine-linked glycosylation (N-glycosylation) of proteins in the cancer secretome has been gaining increasing attention as a potential biomarker for cancer detection and diagnosis. Small extracellular vesicles (sEVs) constitute a large part of the cancer secretome, yet little is known about whether their N-glycosylation status reflects known cancer characteristics. Here, we investigated the N-glycosylation of sEVs released from small-cell lung carcinoma (SCLC) and non–small-cell lung carcinoma (NSCLC) cells. We found that the N-glycans of SCLC-sEVs were characterized by the presence of structural units also found in the brain N-glycome, while NSCLC-sEVs were dominated by typical lung-type N-glycans with NSCLC-associated core fucosylation. In addition, lectin-assisted N-glycoproteomics of SCLC-sEVs and NSCLC-sEVs revealed that integrin αV was commonly expressed in sEVs of both cancer cell types, while the epithelium-specific integrin α6β4 heterodimer was selectively expressed in NSCLC-sEVs. Importantly, N-glycomics of the immunopurified integrin α6 from NSCLC-sEVs identified NSCLC-type N-glycans on this integrin subunit. Thus, we conclude that protein N-glycosylation in lung cancer sEVs may potentially reflect the histology of lung cancers.
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影响因子：
8.8
作者：
Borromeo MD;Savage TK;Kollipara RK;He M;Augustyn A;Osborne JK;Girard L;Minna JD;Gazdar AF;Cobb MH;Johnson JE
通讯作者：
Johnson JE
DOI：
10.1126/science.aau6977
发表时间：
2020-02-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kalluri R;LeBleu VS
通讯作者：
LeBleu VS
影响因子：
21.3
作者：
Costa-Silva B;Aiello NM;Ocean AJ;Singh S;Zhang H;Thakur BK;Becker A;Hoshino A;Mark MT;Molina H;Xiang J;Zhang T;Theilen TM;García-Santos G;Williams C;Ararso Y;Huang Y;Rodrigues G;Shen TL;Labori KJ;Lothe IM;Kure EH;Hernandez J;Doussot A;Ebbesen SH;Grandgenett PM;Hollingsworth MA;Jain M;Mallya K;Batra SK;Jarnagin WR;Schwartz RE;Matei I;Peinado H;Stanger BZ;Bromberg J;Lyden D
通讯作者：
Lyden D
影响因子：
4.6
作者：
Hang Q;Isaji T;Hou S;Zhou Y;Fukuda T;Gu J
通讯作者：
Gu J
影响因子：
2.7
作者：
GU, JG;NISHIKAWA, A;TANIGUCHI, N
通讯作者：
TANIGUCHI, N