MiR-223 targeting MAFB suppresses proliferation and migration of nasopharyngeal carcinoma cells.
MiR-223 targeting MAFB suppresses proliferation and migration of nasopharyngeal carcinoma cells.
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DOI：
10.1186/s12885-015-1464-x
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发表时间：
2015-06-09
期刊：
影响因子：
3.8
通讯作者：
Lu S
中科院分区：
文献类型：
--
作者：
Yang W;Lan X;Li D;Li T;Lu S
越来越多的证据表明miRNA在肿瘤发病机制中发挥重要作用，本研究旨在鉴定候选miRNA并探讨其在鼻咽癌（NPC）中的作用。通过微阵列分析筛选miRNA和mRNA表达。采用MTT法、软琼脂法和伤口愈合实验检测细胞增殖、集落形成和迁移能力。通过裸鼠移植瘤生长抑制来评价肿瘤生长抑制。采用TaqMan法检测鼻咽癌患者血浆中miR-223的水平。采用实时定量PCR和蛋白质印迹法确认miR-223和MAFB的表达水平。使用双荧光素酶报告基因测定验证miR-223和MAFB之间的靶向关系。miR-223在CNE-1、CNE-2细胞中的表达较NP 69细胞（一种永生化人鼻咽上皮细胞系）降低，并且其在NPC患者血浆中的水平也较健康对照降低。miR-223在CNE-2细胞中的外源性表达可抑制CNE-2细胞的体外和体内增殖。外源性miR-223可降低CNE-2细胞集落形成和迁移能力。MAFB是Maf家族成员的转录因子，被鉴定为miR-223的靶基因。我们发现MAFB沉默抑制了迁移和侵袭能力。miR-223通过降低MAFB表达负调控鼻咽癌细胞的生长和迁移，这一发现为理解miR-223在鼻咽癌发生中的调控机制提供了新的视角。
Mounting evidence suggests that miRNAs have major functions in tumor pathogenesis, and this study aimed to identify the candidate miRNA and investigate its role in nasopharyngeal carcinoma (NPC). MiRNA and mRNA expressions were screened by microarray assays. The cell proliferation, colony formation and migration ability were measured by MTT, soft agar and wound healing assays, respectively. The tumor growth suppression was evaluated by xenografting in nude mice. The plasma miR-223 levels in NPC patients were detected by TaqMan analysis. Real-time quantitative PCR and Western blotting were used to confirm miR-223 and MAFB expression levels. The targeting relationship between miR-223 and MAFB was verified using dual luciferase reporter assay. The miR-223 expression was decreased in CNE-1, CNE-2 cells as compared with NP69 cells, an immortalized human nasopharyngeal epithelial cell line, and its level also reduced in NPC patients’ plasma as compared with healthy controls. Exogenous expression of miR-223 in CNE-2 cells could inhibit cell proliferation both in vitro and in vivo. Extrogenous miR-223 in CNE-2 cells would decrease the ability of colony formation and migration. MAFB, a transcription factor of Maf family members, was identified as a target gene of miR-223. We found that migration and invasion abilities were inhibited by MAFB silencing. MiR-223 negatively regulates the growth and migration of NPC cells via reducing MAFB expression, and this finding provides a novel insight into understanding miR-223 regulation mechanism in nasopharyngeal carcinoma tumorigenesis.
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DOI：
10.1158/1078-0432.ccr-13-0601
发表时间：
2013-08-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Streppel MM;Pai S;Campbell NR;Hu C;Yabuuchi S;Canto MI;Wang JS;Montgomery EA;Maitra A
通讯作者：
Maitra A
miR-216b通过靶向KRAS抑制鼻咽癌中的肿瘤生长和侵袭
影响因子：
4
作者：
Deng, Min;Tang, Hailin;Li, Guiyuan
通讯作者：
Li, Guiyuan
影响因子：
11.2
作者：
Jones KB;Salah Z;Del Mare S;Galasso M;Gaudio E;Nuovo GJ;Lovat F;LeBlanc K;Palatini J;Randall RL;Volinia S;Stein GS;Croce CM;Lian JB;Aqeilan RI
通讯作者：
Aqeilan RI
影响因子：
64.8
作者：
Johnnidis, Jonathan B.;Harris, Marian H.;Camargo, Fernando D.
通讯作者：
Camargo, Fernando D.
影响因子：
14.9
作者：
Betel D;Wilson M;Gabow A;Marks DS;Sander C
通讯作者：
Sander C