Potassium-39 nuclear magnetic resonance observation of intracellular potassium without chemical shift reagents during metabolic inhibition in the isolated perfused rat heart.
Potassium-39 nuclear magnetic resonance observation of intracellular potassium without chemical shift reagents during metabolic inhibition in the isolated perfused rat heart.
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离体灌注大鼠心脏代谢抑制过程中不使用化学位移试剂的 Potass-39 核磁共振观察细胞内钾。
DOI：
10.1161/01.res.67.2.401
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发表时间：
1990
影响因子：
20.1
通讯作者：
Y. Kawashima
中科院分区：
文献类型：
--
作者：
S. Kuki;E. Suzuki;H. Watari;H. Takami;H. Matsuda;Y. Kawashima
基于细胞内钾的自旋晶格弛豫时间（T1）（8.45 T 时为 8.3 毫秒）远快于细胞外钾的自旋晶格弛豫时间（T1）（68 毫秒），在 33℃ 下通过钾 39 核磁共振 (NMR) 光谱测量灌注大鼠心脏的细胞内钾含量。代谢抑制（2 mM 氰化物，0 mM 葡萄糖）开始后 40 分钟，细胞内钾降至对照水平的 60.2 +/- 4.3%（平均值 +/- SEM，n = 6）。氧化代谢重新启动后 30 分钟，氰化物的去除使细胞内钾恢复至 94.2 +/- 3.9%。累积钾损失由冠状动脉流出物的流速和钾浓度确定，在40分钟的代谢抑制期间达到139+/-12μmol/g干重。该值计算为对照心脏中细胞内钾的 41.8%，并且与通过 NMR 测量的细胞内钾的减少量一致。在代谢抑制和恢复期间，39K NMR观察到的细胞内钾的变化与累积钾损失之间观察到线性相关。目前的结果评估了反转恢复技术作为成功监测心肌细胞内钾的方法。
The intracellular potassium content of perfused rat heart was measured by potassium-39 nuclear magnetic resonance (NMR) spectroscopy at 33 degrees C with an inversion recovery technique based on the fact that the spin-lattice relaxation time (T1) of the intracellular potassium (8.3 msec at 8.45 T) is much faster than that of the extracellular potassium (68 msec). Intracellular potassium decreased to 60.2 +/- 4.3% of the control level (mean +/- SEM, n = 6) at 40 minutes from the start of metabolic inhibition (2 mM cyanide, 0 mM glucose). Removal of cyanide restored intracellular potassium to 94.2 +/- 3.9% at 30 minutes from the restart of oxidative metabolism. The cumulative potassium loss was determined from the flow rate and potassium concentration of the coronary effluent, which reached 139 +/- 12 mumol/g dry wt during 40 minutes of metabolic inhibition. This value was calculated as 41.8% of intracellular potassium in the control heart and agreed with the decrement of intracellular potassium measured by NMR. During the metabolic inhibition and recovery period, a linear correlation was observed between the changes in 39K NMR-observed intracellular potassium and the cumulative potassium loss. The present results evaluate the inversion recovery technique as a method to successfully monitor the myocardial intracellular potassium.
灌注青蛙心脏细胞内离子的核磁共振研究。
DOI：
10.1152/ajpheart.1987.252.6.h1138
发表时间：
1987
期刊：
The American journal of physiology
影响因子：
--
作者：
Burstein,D;Fossel,ET
通讯作者：
Fossel,ET
分级冠脉流量减少对猪缺血离子、电和机械指标的影响。
影响因子：
37.8
作者：
Watanabe,I;Johnson,TA;Buchanan,J;Engle,CL;Gettes,LS
通讯作者：
Gettes,LS
早期心肌缺血期间[K]o 的积累和电生理变化。
DOI：
10.1152/ajpheart.1982.243.2.h318
发表时间：
1982
期刊：
The American journal of physiology
影响因子：
--
作者：
Weiss,J;Shine,KI
通讯作者：
Shine,KI