Zika Virus Infects Human Placental Macrophages.
Zika Virus Infects Human Placental Macrophages.
复制标题
DOI：
10.1016/j.chom.2016.05.015
复制
发表时间：
2016-07-13
影响因子：
30.3
通讯作者：
Suthar MS
中科院分区：
文献类型：
--
作者：
Quicke KM;Bowen JR;Johnson EL;McDonald CE;Ma H;O'Neal JT;Rajakumar A;Wrammert J;Rimawi BH;Pulendran B;Schinazi RF;Chakraborty R;Suthar MS
最近在巴西爆发的寨卡病毒（ZIKV）与新生儿小头畸形病例的增加直接相关。目前的证据表明，ZIKV从母亲垂直传播给胎儿。然而，宫内传播的机制和涉及的细胞类型仍然未知。我们证明了当代ZIKV毒株PRVABC59（PR 2015）在原代人胎盘巨噬细胞（称为Hofbauer细胞）中感染和复制，并且在较小程度上在从足月胎盘的绒毛组织分离的细胞滋养层中感染和复制。病毒复制与I型干扰素（IFN）、促炎细胞因子和抗病毒基因表达的诱导相一致，但具有最小的细胞死亡。我们的结果表明了子宫内传播的机制，其中ZIKV通过直接感染胎盘细胞并破坏胎盘屏障而进入胎儿室。目前流行的寨卡病毒株与胎儿小头畸形症直接相关。然而，宫内ZIKV传播的机制尚不清楚。Quicke等人证明，当代ZIKV毒株在原代人胎盘巨噬细胞和细胞滋养层中感染和复制，表明ZIKV穿过胎盘屏障的途径。
The recent Zika virus (ZIKV) outbreak in Brazil has been directly linked to increased cases of microcephaly in newborns. Current evidence indicates that ZIKV is transmitted vertically from mother to fetus. However, the mechanism of intrauterine transmission and the cell types involved remain unknown. We demonstrate that the contemporary ZIKV strain PRVABC59 (PR 2015) infects and replicates in primary human placental macrophages, called Hofbauer cells, and to a lesser extent in cytotrophoblasts, isolated from villous tissue of full-term placentae. Viral replication coincides with induction of type I interferon (IFN), pro-inflammatory cytokines, and antiviral gene expression, but with minimal cell death. Our results suggest a mechanism for intra-uterine transmission in which ZIKV gains access to the fetal compartment by directly infecting placental cells and disrupting the placental barrier. The currently circulating Zika virus strain is directly linked to fetal microcephaly. However, the mechanism of intrauterine ZIKV transmission is unknown. Quicke et al. demonstrate that a contemporary ZIKV strain infects and replicates in primary human placental macrophages and cytotrophoblasts, suggesting a route for ZIKV to cross the placental barrier.
登录
查看更多内容
DOI：
10.1126/science.aaf5036
发表时间：
2016-04-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Faria NR;Azevedo RDSDS;Kraemer MUG;Souza R;Cunha MS;Hill SC;Thézé J;Bonsall MB;Bowden TA;Rissanen I;Rocco IM;Nogueira JS;Maeda AY;Vasami FGDS;Macedo FLL;Suzuki A;Rodrigues SG;Cruz ACR;Nunes BT;Medeiros DBA;Rodrigues DSG;Queiroz ALN;da Silva EVP;Henriques DF;da Rosa EST;de Oliveira CS;Martins LC;Vasconcelos HB;Casseb LMN;Simith DB;Messina JP;Abade L;Lourenço J;Alcantara LCJ;de Lima MM;Giovanetti M;Hay SI;de Oliveira RS;Lemos PDS;de Oliveira LF;de Lima CPS;da Silva SP;de Vasconcelos JM;Franco L;Cardoso JF;Vianez-Júnior JLDSG;Mir D;Bello G;Delatorre E;Khan K;Creatore M;Coelho GE;de Oliveira WK;Tesh R;Pybus OG;Nunes MRT;Vasconcelos PFC
通讯作者：
Vasconcelos PFC
影响因子：
30.3
作者：
Welsch S;Miller S;Romero-Brey I;Merz A;Bleck CK;Walther P;Fuller SD;Antony C;Krijnse-Locker J;Bartenschlager R
通讯作者：
Bartenschlager R
影响因子：
33.9
作者：
Martines, Roosecelis Brasil;Bhatnagar, Julu;Zaki, Sherif
通讯作者：
Zaki, Sherif
影响因子：
30.3
作者：
Schulz O;Pichlmair A;Rehwinkel J;Rogers NC;Scheuner D;Kato H;Takeuchi O;Akira S;Kaufman RJ;Reis e Sousa C
通讯作者：
Reis e Sousa C
影响因子：
5.4
作者：
Chiuppesi, Flavia;Wussow, Felix;Diamond, Don J.
通讯作者：
Diamond, Don J.