Whole-body integration of gene expression and single-cell morphology.
Whole-body integration of gene expression and single-cell morphology.
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DOI：
10.1016/j.cell.2021.07.017
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发表时间：
2021-09-02
期刊：
影响因子：
64.5
通讯作者：
Arendt D
中科院分区：
文献类型：
--
作者：
Vergara HM;Pape C;Meechan KI;Zinchenko V;Genoud C;Wanner AA;Mutemi KN;Titze B;Templin RM;Bertucci PY;Simakov O;Dürichen W;Machado P;Savage EL;Schermelleh L;Schwab Y;Friedrich RW;Kreshuk A;Tischer C;Arendt D
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Animal bodies are composed of cell types with unique expression programs that implement their distinct locations, shapes, structures, and functions. Based on these properties, cell types assemble into specific tissues and organs. To systematically explore the link between cell-type-specific gene expression and morphology, we registered an expression atlas to a whole-body electron microscopy volume of the nereid Platynereis dumerilii. Automated segmentation of cells and nuclei identifies major cell classes and establishes a link between gene activation, chromatin topography, and nuclear size. Clustering of segmented cells according to gene expression reveals spatially coherent tissues. In the brain, genetically defined groups of neurons match ganglionic nuclei with coherent projections. Besides interneurons, we uncover sensory-neurosecretory cells in the nereid mushroom bodies, which thus qualify as sensory organs. They furthermore resemble the vertebrate telencephalon by molecular anatomy. We provide an integrated browser as a Fiji plugin for remote exploration of all available multimodal datasets. A cellular atlas integrates gene expression and ultrastructure for an entire annelid Morphometry of all segmented cells, nuclei, and chromatin categorizes cell classes Molecular anatomy and projectome of head ganglionic nuclei and mushroom bodies An open-source browser for multimodal big image data exploration and analysis A framework for integrating cellular-resolution gene expression and cell morphological information at full-organism scale is provided for the marine annelid Platynereis dumerilii
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影响因子：
7.7
作者：
Bezares-Calderon, Luis A.;Berger, Juergen;Jekely, Gaspar
通讯作者：
Jekely, Gaspar
影响因子：
1.5
作者：
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通讯作者：
Baldwin, James G.
影响因子：
5.8
作者：
Chartier, Thomas F.;Deschamps, Joran;Arendt, Detlev
通讯作者：
Arendt, Detlev
影响因子：
10.7
作者：
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通讯作者：
Arendt D
DOI：
10.1088/1742-5468/2008/10/p10008
发表时间：
2008-10-01
影响因子：
2.4
作者：
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通讯作者：
Lefebvre, Etienne