Replication of long-bone length QTL in the F9-F10 LG,SM advanced intercross.
Replication of long-bone length QTL in the F9-F10 LG,SM advanced intercross.
复制标题
DOI：
10.1007/s00335-009-9174-9
复制
发表时间：
2009-04
期刊：
影响因子：
2.5
通讯作者：
Cheverud, James M.
中科院分区：
文献类型：
--
作者：
Norgard, Elizabeth A.;Jarvis, Joseph P.;Roseman, Charles C.;Maxwell, Taylor J.;Kenney-Hunt, Jane P.;Samocha, Kaitlin E.;Pletscher, L. Susan;Wang, Bing;Fawcett, Gloria L.;Leatherwood, Christopher J.;Wolf, Jason B.;Cheverud, James M.
数量性状基因座（QTL）作图技术经常用于鉴定与感兴趣的表型中的变异相关的基因组区域。然而，通常采用的F2互交和同源菌株群体具有有限的遗传分辨率，导致相对较大的置信区间，极大地抑制了统计结果的功能确认。在这里，我们使用LG，SM高级杂交的组合F9和F10代（n= 1，455）的增加的分辨率来精细定位先前鉴定的与肱骨、尺骨、股骨和胫骨的长度相关的QTL。共检测到81个影响长骨长度的QTL。其中，49个先前在该互交的组合F2-F3群体中鉴定，而32个代表性状方差的新贡献者。多效性分析表明，大多数QTL影响3-4个长骨或连续同源肢节。我们还确定了72上位相互作用涉及38个QTL和88个新的区域。该分析表明，使用先进的互交后代极大地促进了置信区间的精细定位，将3个F2-F3 QTL分解为多个连锁位点，并缩小其他位点的置信区间，以及允许鉴定额外的QTL。进一步表征这些QTL的生物学基础将有助于更好地理解长骨长度小变异的遗传学。
Quantitative trait locus (QTL) mapping techniques are frequently used to identify genomic regions associated with variation in phenotypes of interest. However, the F2 intercross and congenic strain populations usually employed have limited genetic resolution resulting in relatively large confidence intervals that greatly inhibit functional confirmation of statistical results. Here, we use the increased resolution of the combined F9 and F10 generations (n=1,455) of the LG,SM advanced intercross to fine-map previously identified QTL associated with the lengths of the humerus, ulna, femur, and tibia. We detected 81 QTL affecting long bone lengths. Of these, 49 were previously identified in the combined F2-F3 population of this intercross while 32 represent novel contributors to trait variance. Pleiotropy analysis suggests that most QTL affect 3-4 long bones or serially homologous limb segments. We also identified 72 epistatic interactions involving 38 QTL and 88 novel regions. This analysis shows that using later generations of an advanced intercross greatly facilitates fine-mapping of confidence intervals, resolving 3 F2-F3 QTL into multiple linked loci and narrowing confidence intervals of other loci, as well as allowing identification of additional QTL. Further characterization of the biological bases of these QTL will help provide a better understanding of the genetics of small variations in long bone length.
登录
查看更多内容
影响因子：
3.8
作者：
HALEY, CS;KNOTT, SA
通讯作者：
KNOTT, SA
影响因子：
2.5
作者：
Corva, PM;Horvat, S;Medrano, JF
通讯作者：
Medrano, JF
影响因子：
3.8
作者：
Li, J;Ji, L
通讯作者：
Ji, L
影响因子：
3.3
作者：
Cheverud, JM;Routman, EJ
通讯作者：
Routman, EJ
影响因子：
3.3
作者：
Kenney-Hunt, Jane P.;Wang, Bing;Cheverud, James M.
通讯作者：
Cheverud, James M.