Comparative genomic analyses of Mycoplasma hyopneumoniae pathogenic 168 strain and its high-passaged attenuated strain.
Comparative genomic analyses of Mycoplasma hyopneumoniae pathogenic 168 strain and its high-passaged attenuated strain.
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猪肺炎支原体病原168株及其高传代减毒株的比较基因组分析
DOI：
10.1186/1471-2164-14-80
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发表时间：
2013-02-05
期刊：
影响因子：
4.4
通讯作者：
Fang L
中科院分区：
文献类型：
--
作者：
Liu W;Xiao S;Li M;Guo S;Li S;Luo R;Feng Z;Li B;Zhou Z;Shao G;Chen H;Fang L
猪肺炎支原体是猪地方性肺炎（EP）的病原体，EP是猪的一种轻度慢性肺炎。尽管猪瘟的直接死亡率很低，但它对养猪业造成了重大的经济损失。鉴定m的毒力相关决定因素。我们确定了肺炎链球菌的全基因组序列。猪肺炎菌株168及其减毒高传代菌株168- l进行比较基因组分析。结果首次对m。并对可能与毒力有关的编码序列（CDSs）进行了初步调查。168- l基因组的基因含量和序列与168非常相似，但由于有60个插入和43个缺失，168- l基因组要小4,483 bp。此外，还鉴定出227个单核苷酸变异（snv）。我们进一步研究了影响cds的变异，并将它们与已报道的毒力决定因素进行了比较。值得注意的是，几乎所有报道的毒力决定因素都包括在这些受影响的cds变异中。除了先前描述的支原体粘附素（P97、P102、P146、P159、P216和LppT）、细胞包膜蛋白（P95）、细胞表面抗原（P36）、分泌蛋白和伴体蛋白（DnaK）的变异外，与代谢和生长相关的基因突变也可能有助于168-L毒力的减弱。此外，许多突变位于先前描述的重复基序中，这可能对毒力至关重要。结论研究了m的毒力衰减机制。通过对168毒株及其高传代弱毒株168- l的比较基因组分析。我们的研究结果提供了可能与毒力有关的cds的初步调查。虽然这些包括报道的毒力相关基因，但也检测到其他新的毒力决定因素。这一新发现将为今后研究多发性硬化症的发病机制奠定基础。并促进新疫苗的设计。
BackgroundMycoplasma hyopneumoniaeis the causative agent of porcine enzootic pneumonia (EP), a mild, chronic pneumonia of swine. Despite presenting with low direct mortality, EP is responsible for major economic losses in the pig industry. To identify the virulence-associated determinants ofM. hyopneumoniae, we determined the whole genome sequence ofM. hyopneumoniaestrain 168 and its attenuated high-passage strain 168-L and carried out comparative genomic analyses.ResultsWe performed the first comprehensive analysis ofM. hyopneumoniae strain 168 and its attenuated strain and made a preliminary survey of coding sequences (CDSs) that may be related to virulence. The 168-L genome has a highly similar gene content and order to that of 168, but is 4,483 bp smaller because there are 60 insertions and 43 deletions in 168-L. Besides these indels, 227 single nucleotide variations (SNVs) were identified. We further investigated the variants that affected CDSs, and compared them to reported virulence determinants. Notably, almost all of the reported virulence determinants are included in these variants affected CDSs. In addition to variations previously described in mycoplasma adhesins (P97, P102, P146, P159, P216, and LppT), cell envelope proteins (P95), cell surface antigens (P36), secreted proteins and chaperone protein (DnaK), mutations in genes related to metabolism and growth may also contribute to the attenuated virulence in 168-L. Furthermore, many mutations were located in the previously described repeat motif, which may be of primary importance for virulence.ConclusionsWe studied the virulence attenuation mechanism ofM. hyopneumoniaeby comparative genomic analysis of virulent strain 168 and its attenuated high-passage strain 168-L. Our findings provide a preliminary survey of CDSs that may be related to virulence. While these include reported virulence-related genes, other novel virulence determinants were also detected. This new information will form the foundation of future investigations into the pathogenesis ofM. hyopneumoniaeand facilitate the design of new vaccines.
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影响因子：
3.6
作者：
Burnett, TA;Dinkla, K;Djordjevic, SP
通讯作者：
Djordjevic, SP
DOI：
10.1099/00221287-139-2-317
发表时间：
1993-02-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
HALDIMANN, A;NICOLET, J;FREY, J
通讯作者：
FREY, J
DOI：
10.1128/cdli.10.3.459-468.2003
发表时间：
2003-05-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Bouh, KCS;Shareck, F;Dea, S
通讯作者：
Dea, S
影响因子：
9.4
作者：
FUTO, S;SETO, Y;MORI, Y
通讯作者：
MORI, Y
影响因子：
14.9
作者：
EMMERT, DB;STOEHR, PJ;CAMERON, GN
通讯作者：
CAMERON, GN