Gut Microbiota in Nymph and Adults of the Giant Mesquite Bug (Thasus neocalifornicus) (Heteroptera: Coreidae) Is Dominated by Burkholderia Acquired De Novo Every Generation
Gut Microbiota in Nymph and Adults of the Giant Mesquite Bug (Thasus neocalifornicus) (Heteroptera: Coreidae) Is Dominated by Burkholderia Acquired De Novo Every Generation
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巨型豆科灌木 (Thasus neocalifornicus)（异翅目：Coreidae）若虫和成虫的肠道微生物群以每一代新获得的伯克霍尔德杆菌为主
DOI：
10.1603/en10309
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
J. X. Becerra
中科院分区：
文献类型：
--
作者：
Sarai Olivier;Zakee L Sabree;Koji Noge;J. X. Becerra
摘要
大牧豆蝽（学名：Thumneocalifornicus Brailovsky and Barrera）是一种生活在美国西南部的昆虫。成虫和成虫经常聚集在牧豆树上（牧豆树属：（豆科）吃豆荚和植物汁液的。我们的特点是土著细菌种群的Escherichia和成年人的这个物种，通过使用分子和系统发育技术和培养方法。结果表明，该昆虫的肠道细菌群落具有有限的多样性占主导地位的伯克霍尔德氏菌associates。系统发育分析表明，这些-Proteobacteria密切相关的共生体从其他异翅目中肠微生物群落，但不伯克霍尔德菌共生体与其他昆虫订单。这些细菌不存在于卵中，也没有在所有年轻的昆虫中发现，这表明它们是在昆虫孵化后获得的。饲养实验表明，如果没有获得这种共生体，巨大的牧豆树错误的经验更高的死亡率。卵，全身DNA提取的年轻的幼虫，和中肠DNA提取的第五龄幼虫和成人也揭示了变形菌属的沃尔巴克氏体的存在。然而，这种细菌也存在于成年人的生殖器官中，表明这种共生体不是肠道特有的。
ABSTRACT
The coreid bug Thasus neocalifornicus Brailovsky and Barrera, commonly known as the giant mesquite bug, is a ubiquitous insect of the southwestern United States. Both nymphs and adults are often found aggregated on mesquite trees (Prosopis spp.: Fabaceae) feeding on seedpods and plant sap. We characterized the indigenous bacterial populations of nymphs and adults of this species by using molecular and phylogenetic techniques and culturing methods. Results show that this insect's bacterial gut community has a limited diversity dominated by Burkholderia associates. Phylogenetic analysis by using 16s rRNA sequences suggests that these &bgr;-Proteobacteria are closely related to those symbionts obtained from other heteropteran midgut microbial communities but not to Burkholderia symbionts associated with other insect orders. These bacteria were absent from the eggs and were not found in all younger nymphs, suggesting that they are acquired after the insects have hatched. Rearing experiments of nymphs with potentially Burkholderia contaminated soil suggested that if this symbiont is not acquired, giant mesquite bugs experience higher mortality. Egg, whole-body DNA extractions of younger nymphs, and midgut DNA extractions of fifth-instar nymphs and adults also revealed the presence of &agr;-Proteobacteria from the Wolbachia genus. However, this bacterium was also present in reproductive organs of adults, indicating that this symbiont is not specific to the gut.
影响因子：
3.8
作者：
Dobson, SL;Bourtzis, K;O'Neill, SL
通讯作者：
O'Neill, SL