Thylakoid DeltapH-dependent precursor proteins bind to a cpTatC-Hcf106 complex before Tha4-dependent transport.
Thylakoid DeltapH-dependent precursor proteins bind to a cpTatC-Hcf106 complex before Tha4-dependent transport.
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依赖于Tha4依赖性转运之前的类囊体三个依赖性前体蛋白与CPTATC-HCF106复合物结合。
DOI：
10.1083/jcb.200105149
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发表时间：
2001-08-20
影响因子：
7.8
通讯作者：
Mori, H
中科院分区：
文献类型：
--
作者：
Cline, K;Mori, H
类囊体ΔpH-dependent途径运输含有双精氨酸信号肽的折叠蛋白。已确定的机械成分包括cpTatC， Hcf106和th4。该反应分两步进行：前体与机器结合，并通过膜运输。在这里，我们发现cpTatC-Hcf106复合物作为特异性结合双精氨酸前体的受体。Hcf106或cpTatC抗体抑制前体结合，但Tha4抗体不抑制。蓝色天然凝胶电泳和洋地黄苷溶解类囊体的共免疫沉淀表明，Hcf106和cpTatC是缺乏Tha4的~ 700-kD复合物的成员。类囊体结合的前体蛋白也与~ 700-kD复合物相关，并与cpTatC或Hcf106抗体共免疫沉淀。化学交联表明前体与cpTatC和Hcf106直接接触，证实了th4在膜中与前体、cpTatC或Hcf106不相关。前体结合到cpTatC-Hcf106复合物需要双精氨酸和信号肽的疏水核心。即使在ΔpH存在的情况下，当Tha4被抗体隔离时，前体仍然与复合物结合。这些结果表明，与cpTatC-Hcf106复合物结合的前体构成了该途径的识别事件，随后Tha4的参与导致易位。
The thylakoid ΔpH-dependent pathway transports folded proteins with twin arginine–containing signal peptides. Identified components of the machinery include cpTatC, Hcf106, and Tha4. The reaction occurs in two steps: precursor binding to the machinery, and transport across the membrane. Here, we show that a cpTatC–Hcf106 complex serves as receptor for specific binding of twin arginine–containing precursors. Antibodies to either Hcf106 or cpTatC, but not Tha4, inhibited precursor binding. Blue native gel electrophoresis and coimmunoprecipitation of digitonin-solubilized thylakoids showed that Hcf106 and cpTatC are members of an ∼700-kD complex that lacks Tha4. Thylakoid-bound precursor proteins were also associated with an ∼700-kD complex and were coimmunoprecipitated with antibodies to cpTatC or Hcf106. Chemical cross-linking revealed that precursors make direct contact with cpTatC and Hcf106 and confirmed that Tha4 is not associated with precursor, cpTatC, or Hcf106 in the membrane. Precursor binding to the cpTatC–Hcf106 complex required both the twin arginine and the hydrophobic core of the signal peptide. Precursors remained bound to the complex when Tha4 was sequestered by antibody, even in the presence of ΔpH. These results indicate that precursor binding to the cpTatC–Hcf106 complex constitutes the recognition event for this pathway and that subsequent participation by Tha4 leads to translocation.
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针对叶绿体类囊体膜中SEC和Delta pH蛋白传输系统的决定因素和提议的进化基础。
DOI：
10.1083/jcb.136.4.823
发表时间：
1997-02-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
Henry R;Carrigan M;McCaffrey M;Ma X;Cline K
通讯作者：
Cline K
影响因子：
64.5
作者：
GORLICH, D;RAPOPORT, TA
通讯作者：
RAPOPORT, TA
影响因子：
3.5
作者：
Mori, H;Summer, EJ;Cline, K
通讯作者：
Cline, K
影响因子：
4.8
作者：
Rodrigue, A;Chanal, A;Wu, LF
通讯作者：
Wu, LF
影响因子：
11.4
作者：
ARKOWITZ, RA;JOLY, JC;WICKNER, W
通讯作者：
WICKNER, W